清痹颗粒的HPLC指纹图谱研究 点击下载
| 论文标题： | 清痹颗粒的HPLC指纹图谱研究 |
| 英文标题： | |
| 中文摘要： | 目的：建立清痹颗粒的高效液相色谱（HPLC）指纹图谱。方法：采用HPLC法。色谱柱为Diamonsil C18，流动相为甲醇-0.3％磷酸溶液（梯度洗脱），流速为1.0 mL/min，检测波长为260 nm，柱温为25 ℃，进样量为10 μL。以黄芩苷为参照，测定10批样品的HPLC图谱，采用《中药色谱指纹图谱相似度评价系统》（2.0版）进行共有峰指认和相似度评价。结果：10批清痹颗粒的HPLC图谱有29个共有峰，相似度均＞0.90。经验证，10批样品HPLC图谱与对照指纹图谱具有较好的一致性。结论：该研究所建指纹图谱可为清痹颗粒的鉴别和质量评价提供参考。 |
| 英文摘要： | OBJECTIVE： To establish HPLC fingerprint of Qingbi granules. METHODS： HPLC method was adopted. The determination was performed on Diamonsil C18 column with mobile phase consisted of methanol-0.3％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 260 nm， and column temperature was 25 ℃. The sample size was 10 μL. Using baicalin as reference， HPLC chromatograms of 10 batches of samples were determined. Common peak identification and similarity evaluation were performed by using TCM Chromatographic Fingerprint Similarity Evaluation Software （2.0 edition）. RESULTS： There were 29 common peaks in HPLC chromatograms of 10 batches of samples. The similarity among the 10 batches was more than 0.90. After validation， HPLC chromatograms of 10 batches of samples were in line with control fingerprints. CONCLUSIONS： Established fingerprints can provide reference for identification and quality evaluation of Qingbi granules. |
| 期刊： | 2017年第28卷第30期 |
| 作者： | 白雪洁，于淼，刘秀华，白雪媛，李庆杰 |
| 英文作者： | BAI Xuejie，YU Miao，LIU Xiuhua，BAI Xueyuan，LI Qingjie |
| 关键字： | 清痹颗粒；指纹图谱；高效液相色谱法 |
| KEYWORDS： | Qingbi granules； Fingerprint； HPLC |
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| 文件大小： | 619.60Kb |
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