苗药黑骨藤多指标的含量测定及聚类分析 点击下载
| 论文标题： | 苗药黑骨藤多指标的含量测定及聚类分析 |
| 英文标题： | |
| 中文摘要： | 目的：建立同时测定苗药黑骨藤中绿原酸和杠柳毒苷含量的方法。方法：采用高效液相色谱法。色谱柱为Xtimate C18，流动相为乙腈-0.1％磷酸溶液（梯度洗脱），流速为1.0 mL/min，检测波长为220 nm，柱温为25 ℃，进样量为10 μL。采用SPSS 23.0统计软件根据药材样品中绿原酸和杠柳毒苷的含量进行聚类分析。结果：绿原酸、杠柳毒苷检测进样量线性范围分别为0.040 6～1.8 μg（r＝0.999 4）、0.016 8～2.3 μg（r＝0.999 9）；精密度、稳定性、重复性试验的RSD均小于5.0％；定量限分别为0.918 0、0.084 3 μg/mL，检测限分别为0.102 0、0.025 3 μg/mL；加样回收率分别为102.66％～104.00％（RSD＝0.53％，n＝6）、96.44％～100.79％（RSD＝1.73％，n＝6）；耐用性试验的RSD均小于3.0％。18份药材样品可聚为三大类，即S2、S4、S10、S12、S14～S18聚为一类，S1、S3、S5～S7、S9、S11、S13聚为一类，S8为一类。结论：该方法可用于黑骨藤药材的质量控制和品质评价；不同产地黑骨藤药材中绿原酸和杠柳毒苷的含量有较大差异，各成分含量与产地有一定关联。 |
| 英文摘要： | OBJECTIVE： To establish the method for content determination of chlorogenic acid and periplocin in Miao medicine Periploca forrestii. METHODS： HPLC method was adopted. The determination was performed on Xtimate C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 220 nm， and column temperature was maintained at 25 ℃. The sample size was 10 μL. Cluster analysis was conducted according to the content of chlorogenic acid and periplocin in samples by SPSS 23.0 software. RESULTS： The linear range of chlorogenic acid and periplocin were 0.040 6-1.8 μg（r＝0.999 4） and 0.016 8-2.3 μg（r＝0.999 9）， respectively. RSDs of precision， stability and repeatability tests were all lower than 5.0％. The quantitation limits were 0.918 0， 0.084 3 μg/mL， and detection limits were 0.102 0， 0.025 3 μg/mL， respectively. RSD of durability were lower than 3.0％. The recoveries were 102.66％-104.00％（RSD＝0.53％，n＝6）， 96.44％-100.79％（RSD＝1.73％，n＝6），respectively. RSD of durability were lower than 3.0％. The result indicated that 18 batches of samples were divided into 3 categories by cluster analysis. S2， S4， S10， S12 and S14-S18 were divided into one category； S1， S3， S5-S7， S9， S11 and S13 were divided into another category； S8 was regarded as one category. CONCLUSIONS： The method can be applied for quality control and evaluation of P. forrestii. The contents of chlorogenic acid and periplocin in P. forrestii from different producing areas are different greatly. There is a certain correlation between the content of each component and the producing area. |
| 期刊： | 2018年第29卷第12期 |
| 作者： | 刘育辰，刘丽苹，刘刚，金文渊，龙毅，黄明喆，杨欣 |
| 英文作者： | LIU Yuchen，LIU Liping，LIU Gang，JIN Wenyuan，LONG Yi，HUANG Mingzhe，YANG Xin |
| 关键字： | 黑骨藤；绿原酸；杠柳毒苷；含量测定；聚类分析 |
| KEYWORDS： | Periploca forrestii； Chtorogenic acid； Periplocin； Content determination； Cluster |
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