内毒素亲和吸附剂SPV对失血性休克模型大鼠肠壁通透性与细菌移位的影响 点击下载
| 论文标题： | 内毒素亲和吸附剂SPV对失血性休克模型大鼠肠壁通透性与细菌移位的影响 |
| 英文标题： | |
| 中文摘要： | 目的：观察内毒素亲和吸附剂SPV对失血性休克模型大鼠肠壁通透性与细菌移位的影响。方法：将85只雄性SD大鼠随机分为正常组（5只）、休克组（每时间点各5只，共20只）以及SPV低、中、高剂量组（将蒙脱石散0.3 g、硫酸多黏菌素B 0.5 mg、维生素B6 5 mg溶于生理盐水中制成总体积为5 mL的SPV溶液，即为低剂量；中、高剂量药物成分是低剂量的2、3倍。各剂量组每时间点各5只，共60只），各给药组大鼠分别灌胃SPV溶液5、10、15 mL，正常组和休克组大鼠灌胃生理盐水5 mL，均给药1次。末次给药30 min后，除正常组外其余各组大鼠均通过股动脉插管放血术复制失血性休克模型。于复苏后1、4、8、16 h分别检测各组大鼠血清中二胺氧化酶（DAO）、内毒素、D-乳酸的活性或含量以及计算肠道细菌移位阳性率。结果：与正常组比较，休克组大鼠各时间点血清DAO活性均显著增强，血清内毒素、D-乳酸的含量以及肠道细菌移位阳性率均显著升高（P＜0.05）。与休克组比较，SPV各剂量组大鼠血清DAO活性（1～16 h各时间点）均显著减弱，血清内毒素、D-乳酸的含量（1～16 h各时间点）以及肠道细菌移位阳性率（SPV低剂量组4～16 h各时间点，SPV中、高剂量组1～16 h各时间点）均显著降低（P＜0.05），且SPV中、高剂量组上述指标（1～16 h各时间点）均显著低于SPV低剂量组（P＜0.05）；而SPV中、高剂量组上述指标组间比较差异均无统计学意义（P＞0.05）。结论：内毒素亲和吸附剂SPV可剂量依赖性地改善失血性休克模型大鼠的肠壁通透性，并抑制细菌移位。这种作用与其降低血清内毒素、DAO、D-乳酸的活性或含量，下调肠道细菌移位阳性率有关。 |
| 英文摘要： | OBJECTIVE： To observe the effects of endotoxin affinity adsorbent SPV on intestinal permeability and bacterial translocation in hemorrhagic shock model rats. METHODS： Totally 85 male SD rats were randomly divided into normal group （5 rats）， shock group （each 5 rats at each time point， 20 rats in total）， SPV low-dose， medium-dose and high-dose groups （Montmorillonite powder 0.3 g， Polymyxin B sulfate 0.5 mg， Vitamin B6 5 mg dissolved in normal saline to obtain SPV solution 5 mL， as low dose； medium and high dose were 2 or 3 times as high as low dose. Each 5 rats of each group at each time point， 60 rats in total）. Administration groups were given SPV solution intragastrically 5， 10， 15 mL once， respectively； normal group and shock group were given normal saline 5 mL intragastrically once. Thirty minutes after last medication， other groups received femoral artery catheterization and bleeding to induce hemorrhagic shock model， except for normal group. The activities or contents of diamine oxidase （DAO）， endotoxin and D-lactic acid， positive rates of intestinal bacterial translocation were detected in each group at 1， 4， 8， 16 h after recovery. RESULTS： Compared with normal group， the activities of DAO of rats in shock group were enhanced significantly， and the serum contents of endotoxin and D-lactic acid were increased significantly （P＜0.05）. Compared with shock group， the activities of DAO were decreased significantly in SPV groups （at each time point during 1-16 h）； the serum contents of endotoxin and D-lactic acid （at each time point during 1-16 h）， positive rates of intestinal bacterial translocation （SPV low-dose group at each time point during 4-16 h， SPV medium-dose and high-dose groups at each time point during 1-16 h） were decreased significantly （P＜0.05）. Above indexes in SPV medium-dose and high-dose groups （at each time point during 1-16 h） were significantly lower than those of SPV low-dose group （P＜0.05）. There was no statistical significance in above indexes between SPV medium-dose group and high-dose group （P＞0.05）. CONCLUSIONS： The endotoxin affinity adsorbent SPV can improve the permeability of the intestinal wall and inhibit bacterial translocation in hemorrhagic shock model rats in dose-dependent manner. The effects of which may be associated with reducing the activities or contents of serum DAO， endotoxin， D-lactic acid， and down-regulating the positive rate of bacterial translocation. |
| 期刊： | 2019年第30卷第2期 |
| 作者： | 刘海 |
| 英文作者： | LIU Hai |
| 关键字： | 失血性休克；二胺氧化酶；内毒素；D-乳酸；肠壁通透性；细菌移位；大鼠 |
| KEYWORDS： | Hemorrhagic shock； Diamine oxidase； Endotoxin； D-lactic acid； Intestinal permeability； Bacterial translocation； Rats |
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