车前子及其混淆品的微观特征荧光鉴别研究 点击下载
| 论文标题： | 车前子及其混淆品的微观特征荧光鉴别研究 |
| 英文标题： | |
| 中文摘要： | 目的：建立车前子及其混淆品的微观特征荧光鉴别方法，为中药饮片的市场监管及检验提供技术支持。方法：应用荧光体式显微镜分别在可见光和紫外光下观察，从整体表面特征、种脐特征和切面特征3个方面对车前子[车前（Platago asiatica L.）、平车前（Platago depressa Willd.）的种子]及其混淆品大车前（Platago major L.）的种子、荆芥（Schizonepeta tenuifolia Briq.）的果实、党参[Codonopsis pilosula （Franch.） Nannf.]的种子、地肤[Kochia scoparia（L.） Schrad.]的种子（去皮）、北柴胡（Bupleurum chinense DC.）的果实进行比较研究和鉴别。结果：在可见光下观察，正品车前子表面纹理为波状条纹或细皱纹，混淆品分别为波状条纹、纵棱或纹理不明显；正品车前子种脐位于腹面中央，混淆品除大车前子外其余位于一端；正品车前子切面可见明显的胚直生其中，混淆品除大车前子外其余胚细小或呈环形。在紫外光下观察，正品车前子表面波状条纹明显，呈橙色与浅蓝绿色相间的荧光，平车前表面为网格状皱纹，呈灰蓝色与灰棕色相间的荧光；正品车前子种脐荧光强烈，混淆品中除荆芥果外其余荧光微弱；正品车前子与混淆品切面在荧光颜色、胚大小及分布位置上均有明显差异。结论：荧光体式显微技术可有效、准确地鉴别车前子药材的真伪。 |
| 英文摘要： | OBJECTIVE： To establish a fluorescence identification method of the microscopic characteristics for Plantagin Semen and its adulterants， and to provide technical support for the market supervision and inspection of TCM decoction pieces. METHODS： Under visible and ultraviolet light， comparative study and identification of the Plantagin Semen （seeds of Platago asiatica L. and Platago depressa Willd.） and its adulterants as seeds of Platago major L.， fruits of Schizonepeta tenuifolia Briq.， seeds of Codonopsis pilosula （Franch.） Nannf， seeds （peeled） of Kochia scoparia （L.） Schrad.， fruits of Bupleurum chinense DC. were carried out by means of stereoscopic fluorescence microscopy from aspects of overall surface characteristics， umbilicus characteristics and section characteristics. RESULTS： Under visible light， the surface texture of Plantagin Semen was wavy stripe or fine wrinkle， while the adulterants were wavy stripe， longitudinal edge or texture was not obvious. The umbilicus of Plantagin Semen was located in the center of the ventral surface， while that of adulterants were located at one end except for P. major. In the section of Plantagin Semen， there were obvious direct embryos， in which the adulterants were small or circular embryos except for P. major. Under ultraviolet light， P. asiatica had obvious wavy stripes in surface， orange and light blue-green fluorescence； P. depressa had grid-shaped wrinkles and gray-blue and gray-brown fluorescence； the umbilical fluorescence of Plantagin Semen was strong， and the fluorescence of the adulterants was weak except for S. tenuifolia. There were obvious differences in fluorescence color， embryo size and distribution between the section of Plantagin Semen and adulterants. CONCLUSIONS： The stereoscopic fluorescence microscopy is effect and accurate for the identification of Plantagin Semen. |
| 期刊： | 2019年第30卷第5期 |
| 作者： | 罗晋萍，宁红婷，郭景文，康帅，张南平，连超杰，马双成 |
| 英文作者： | LUO Jinping，NING Hongting，GUO Jingwen，KANG Shuai，ZHANG Nanping，LIAN Chaojie，MA Shuangcheng |
| 关键字： | 荧光体式显微技术；车前子；混淆品；微观特征；整体表面特征；种脐特征；切面特征 |
| KEYWORDS： | Stereoscopic fluorescence microscopy； Plantagin Semen； Adulterants； Microscopic characteristics； Overall surface characteristics； Umbilicus characteristics； Section characteristics |
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