枸杞类胡萝卜素指纹图谱的建立及其抗氧化活性谱效关系研究 点击下载
| 论文标题： | 枸杞类胡萝卜素指纹图谱的建立及其抗氧化活性谱效关系研究 |
| 英文标题： | |
| 中文摘要： | 目的 建立枸杞类胡萝卜素高效液相色谱（HPLC）指纹图谱，并考察其共有峰与抗氧化活性的谱效关系。方法采用HPLC法进行检测，根据《中药指纹图谱相似度评价系统》（2012版）建立34批不同产地枸杞类胡萝卜素的指纹图谱，并进行相似度评价和共有峰指认。以1，1-二苯基-2-三硝基苯肼（DPPH）自由基清除率为指标，考察枸杞类胡萝卜素的体外抗氧化活性，并采用灰色关联度法分析枸杞类胡萝卜素各共有峰与抗氧化活性的谱效关系。结果34批枸杞类胡萝卜素共有4个共有峰，相似度均不低于0.903；并指认出1号峰为玉米黄质、4号峰为玉米黄质双棕榈酸酯。其对DPPH自由基的清除率为1.792％～3.160％。枸杞类胡萝卜素各共有峰与DPPH自由基清除率均呈正相关，关联度均大于0.6；2号峰、4号峰（玉米黄质双棕榈酸酯）与DPPH自由基清除率的关联度均大于0.8。根据关联度确定各共有峰对DPPH自由基清除率的贡献大小依次为2号峰＞4号峰（玉米黄质双棕榈酸酯）＞1号峰（玉米黄质）＞3号峰。结论本研究成功建立了枸杞类胡萝卜素的HPLC指纹图谱，指认了2个共有峰，其中2号峰所代表化学成分和玉米黄质棕榈酸酯可能是枸杞类胡萝卜素抗氧化活性的物质基础。 |
| 英文摘要： | OBJECTI VE To establish the high performan ce liquid c hromatography（HPLC）fingerprint of carotenoid in Lycium barbarum，and to investigate the spectrum-effect relationship between its common peak and antioxidant activity. METHODS HPLC method was adopted. The fingerprints of carotenoid in 34 batches of L. barbarum from different producing areas were established by Similarity Evaluation System of TCM Fingerprint （2012 edition），and similarity evaluation and common peak identification were carried out. Taking scavenging rate of DPPH free radical as index ，in vitro antioxidant activity of carotenoid in L. barbarum was investigated. The spectrum-effect relationship between the common peaks of carotenoids in L. barbarum and antioxidant activity was analyzed by grey correlation method. RESULTS There were 4 common peaks in the fingerprints of carotenoids in 34 batches of L. barbarum ，and the similarity was not less than 0.903. Peak 1 was identified as zeaxanthin ，and peak 4 as zeaxanthin dipalmitate. The scavenging rates of them to DPPH free radical were 1.792％-3.160％. The common peaks of carotenoids in L. barbarum were positively correlated with scavenging rate of DPPH free radical ，and the correlation degree was greater than 0.6；the correlation degree of peak 2 and peak 4（zeaxanthin dipalmitate ）with scavenging rate of DPPH free radical was greater than 0.8. According to the correlation degree ，the contribution of each common peak to scavenging rate of DPPH free radical was determined as peak 2＞ peak 4（zeaxanthin dipalmitate ）＞peak 1（zeaxanthin）＞peak 3. CONCLUSIONS In this study ，HPLC fingerprint of carotenoid in L. barbarum is successfully established ，and two common peaks are identified. The chemical components represented by peak 2 and zeaxanthin palmitate may be the material basis of antioxidant activity of carotenoid in L. barbarum . |
| 期刊： | 2022年第33卷第05期 |
| 作者： | 王静，王杰，阿娜尔，李彤，杨冬平，戴俊东，董玲 |
| 英文作者： | WANG Jing，WANG Jie，Anaer，LI Tong，YANG Dongping ，DAI Jundong ，DONG Ling |
| 关键字： | 枸杞；类胡萝卜素；指纹图谱；谱效关系 |
| KEYWORDS： | Lycium barbarum ；carotenoid；fingerprint；spectrum-effect relationship |
| 总下载数： | 81次 |
| 本日下载数： | 2次 |
| 本月下载数： | 81次 |
| 文件大小： | 619.60Kb |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！