知母活性成分对脓毒症性心肌损伤模型小鼠的改善作用 点击下载
| 论文标题： | 知母活性成分对脓毒症性心肌损伤模型小鼠的改善作用 |
| 英文标题： | |
| 中文摘要： | 目的 为阐明知母改善脓毒症性心肌损伤的作用及可能的活性物质基础提供实验参考。方法采用热回流法制备知母水提物，大孔吸附树脂法分离知母总双苯吡酮和总皂苷。采用腹腔注射脂多糖建立脓毒症性心肌损伤小鼠模型，考察知母3个提取部位及知母单体成分芒果苷、知母皂苷AⅢ、知母皂苷BⅡ对模型小鼠存活率的影响；HE染色法观察知母单体成分芒果苷、知母皂苷AⅢ、知母皂苷BⅡ对模型小鼠心肌组织形态的影响；检测芒果苷对模型小鼠心肌组织中炎症因子[白细胞介素6（IL-6）、IL-1β和肿瘤坏死因子α（TNF-α）]mRNA表达及活性氧（ROS）水平的影响。结果知母总双苯吡酮、总皂苷、水提物干预组小鼠存活率较模型组均不同程度升高，其中以知母总双苯吡酮部位效果较佳；芒果苷、知母皂苷AⅢ和知母皂苷BⅡ均可改善模型小鼠心肌细胞肿胀、肌束排列紊乱程度，且以芒果苷的效果更为明显。芒果苷干预后小鼠心肌组织中IL-6、IL-1β、TNF-αmRNA表达水平及ROS水平较模型组不同程度降低。结论知母不同提取部位均可提高脓毒症性心肌损伤小鼠的存活率，其中以知母总双苯吡酮部位效果较佳。3个知母单体成分中以芒果苷改善脓毒症性心肌损伤的效果最好，其可能是通过抗炎、抗氧化发挥的作用。 |
| 英文摘要： | OBJECTIVE To provide reference for clarifying improvement effects of Anemarrhena asphodeloides on sepsis- induced myocardial injury and potential material basis. METHODS Water extract of A. asphodeloides was extracted by thermal reflux method. Total xanthone and total saponins in A. asphodeloides were separated by macroporous adsorption resin. The mice model of sepsis-induced myocardial injury was established by intraperitoneal injection of lipopolysaccharide. The effects of the location of three extraction fractions and the monomers of A. asphodeloides as mangiferin， timosaponin AⅢ and timosaponin BⅡ on the survival rate of the model mice were explored. HE staining was used to observe the effects of mangiferin， timosaponin AⅢ and timosaponin BⅡ on myocardial morphology in model mice. The effects of mangiferin on mRNA expressions of inflammatory cytokines [interleukin-6 （IL-6）， IL-1β and tumor necrosis factor-α （TNF-α）] and the level of reactive oxygen species （ROS） in myocardial tissue of model mice were detected. RESULTS Compared with the model group， the survival rate of mice in the intervention group of total xanthone， total saponins and water extract was increased to different extents， especially total xanthone fraction. Mangiferin， timosaponin AⅢ and timosaponin BⅡcould improve the degree of myocardial cell swelling and muscle bundle arrangement disorder in model mice， especially mangiferin. Compared with model group， mRNA expressions of IL-6， IL- 1β and TNF- α， ROS level in myocardium of mice after mangiferin intervention were decreased to different extents. CONCLUSIONS The different extraction fractions of A. asphodeloides can improve survival rate of mice with sepsis-induced myocardial injury， especially total xanthone fraction. Mangiferin is the best among the three monomers of A. asphodeloides to improve sepsis-induced myocardial injury， which may play a role in anti-sepsis myocardial injury by anti-inflammation and antioxidantion. |
| 期刊： | 2023年第34卷第01期 |
| 作者： | 李倩；陈香茗；常鑫鑫；崔文华；李阳；薛紫鲸；郑玉光；宋军娜 |
| 英文作者： | LI Qian， CHEN Xiangming，CHANG Xinxin，CUI Wenhua，LI Yang，XUE Zijing，ZHENG Yuguang，SONG Junna |
| 关键字： | 知母；总双苯吡酮；总皂苷；活性成分；芒果苷；脓毒症性心肌损伤 |
| KEYWORDS： | Anemarrhenae asphodeloides； total xanthone； |
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