基于抗氧化活性的龙眼核标准汤剂提取工艺研究 点击下载
论文标题: 基于抗氧化活性的龙眼核标准汤剂提取工艺研究
英文标题:
中文摘要: 目的 优化龙眼核标准汤剂的提取工艺。方法以加水量、提取时间、提取次数为影响因素,出膏率、没食子酸含量、柯里拉京含量、鞣花酸含量、1,1-二苯基-2-苦基肼(DPPH)自由基半数清除浓度(IC50)和2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS)自由基IC50为评价指标,运用层次分析法(AHP)、基于指标相关性的权重赋权系数(CRITIC)法、AHP-CRITIC法计算各指标权重,结合单因素实验和正交实验优化龙眼核标准汤剂提取工艺,并进行验证。结果根据AHP-CRITIC法确定上述各指标的权重依次为9.224%、11.784%、19.320%、11.206%、20.597%、27.869%。龙眼核标准汤剂的最佳提取工艺为第1次提取加14倍水,提取30min;第2、3次提取均加12倍水,提取20min。3次验证实验所得综合得分平均值为97.96分,RSD为0.97%(n=3)。结论优选的工艺稳定可行,可为龙眼核标准汤剂的进一步开发和利用提供实验依据。
英文摘要: OBJECTIVE To optimize the extraction process of standard decoction of Longan seed. METHODS The amount of water added, extraction time and extraction times as the influencing factors, using the extract yield, gallic acid content, corilagin content, ellagic acid content, half clearance concentration (IC50) of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical and IC50 of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS) free radical as the evaluation indexes, the analytic hierarchy process (AHP), criteria importarue though inter-criteria correlation (CRITIC) and AHP-CRITIC method were used to calculate the weight values of each index; the extraction process of Longan seed standard decoction was optimized by single factor test combined with orthogonal experiment; validation test was also performed. RESULTS According to the AHP-CRITIC method, the weights of the above indicators were determined to be 9.224%, 11.784%, 19.320%, 11.206%, 20.597%, 27.869%, respectively. The best process of standard decoction of longan seed was to add 14 times water for the first extraction and extract for 30 minutes; and 12 times water for the second and third extractions, and extract for 20 minutes. Average comprehensive score of the 3 times validation experiments was 97.96 and the RSD was 0.97% (n=3). CONCLUSIONS The process is simple to operate, stable and feasible, which can provide a experimental basis for the further development and utilization of standard decoction of longan seed.
期刊: 2025年第36卷第03期
作者: 蒋佳丽;王艺洁;谢谭芳;王志萍
英文作者: JIANG Jiali,WANG Yijie,XIE Tanfang,WANG Zhiping
关键字: 龙眼核;标准汤剂;抗氧化活性;正交实验;提取工艺
KEYWORDS: Longan seed; standard decoction; antioxidant activity; orthogonal experiment; extraction process
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