目的：改进2010 年版《中国药典》所载远志中远志酮Ⅲ含量测定的方法。方法：采用高效液相色谱法。色谱柱为

Hypersil BDS C18，流动相为乙腈-0.05％磷酸（梯度洗脱），流速为1.0 ml/min，检测波长为320 nm，柱温为25 ℃，进样量为10 μl。结

果：远志酮Ⅲ检测质量浓度线性范围为0.029～0.928 μg/ml（r＝0.999 1）；精密度、稳定性、重复性试验的RSD＜2％；加样回收率

为94.66％～100.90％（RSD＝2.46％，n＝6）。结论：该方法操作简便、稳定、重复性好，虽测定时间有所延长，但提高了测定的准确

度，更适用于远志中远志酮Ⅲ的含量测定。

OBJECTIVE：To improve the content determination for polygala xanthone Ⅲ in Polygalae Radix contained in Chinese

Pharmacopoeia（2010 edition）. METHODS：HPLC was performed on the column of Hypersil BDS C18 with mobile phase of

acetonitrile-0.05％ phosphoric acid（gradient elution）at a flow rate of 1.0 ml/min；detection wavelength was 320 nm，column temperature

was 25 ℃，and the injection volume was 10 μl. RESULTS：The linear range of polygala xanthone Ⅲ was 0.029-0.928 μg/ml

（r＝0.999 1）；RSDs of precision，stability and reproducibility tests were lower than 2.0％；recovery was 94.66％-100.90％（RSD＝

2.46％，n＝6）. CONCLUSIONS：The method is simple，stable and reproducible. Although the determination time is prolonged，it

has improved the accuracy and it is more suitable for the content determination of polygala xanthone Ⅲ in Polygalae Radix.