Box-Behnken响应面法结合多指标综合加权法优选升麻中酚酸类成分的提取工艺 点击下载
论文标题: Box-Behnken响应面法结合多指标综合加权法优选升麻中酚酸类成分的提取工艺
英文标题:
中文摘要: 目的:优化升麻中酚酸类成分的提取工艺。方法:在单因素试验的基础上,以乙醇体积分数、回流提取时间和液料比3个因素为自变量,以咖啡酸、阿魏酸、异阿魏酸含量和浸出物得率的综合评分为因变量,采用基于Box-Behnken设计的响应面法优化升麻中酚酸类成分的提取工艺。结果:最优提取工艺为以8倍药材量的70%乙醇提取2次,每次200 min。验证试验结果表明,综合评分指标的预测值与实测值相对偏差仅为2.20%。结论:优选的提取工艺方法简单、稳定可行,可为后续升麻药材的制剂生产提供参考。
英文摘要: OBJECTIVE: To optimize the extraction technology of phenolic acids from Cimicifugae foetidae. METHODS: Based on single factor test, the extraction technology of phenolic acids from C. foetidae was optimized by response surface methodology based on Box-Behnken design using ethanol volume fraction, reflux extraction time and liquid to solid ratio as independent variables, comprehensive score of the content of caffeic acid, ferulic acid and isoferulic and extract yield as dependent variable. RESULTS: The optimal extraction technology was as follows as 8-fold 70% ethanol, extracting for 2 times, 200 min each time. Results of validation test indicated that the difference between predicted value of comprehensive score indicators and measured value was 2.20%. CONCLUSIONS: The optimal extraction technology is simple, stable and feasible, and can provide reference for following production of C. foetidae preparation.
期刊: 2016年第27卷第13期
作者: 范孟雪,秦昆明,丁斐,黄雨婷,蔡宝昌
英文作者: FAN Mengxue,QIN Kunming,DING Fei,HUANG Yuting,CAI Baochang
关键字: 升麻;咖啡酸;阿魏酸;异阿魏酸;含量;浸出物得率;Box-Behnken设计;响应面法;提取工艺
KEYWORDS: Cimicifugae foetidae; Caffeic acid; Ferulic acid; Isoferulic acid; Content; Extract yield; Box-Behnken design; Response surface methodology; Extraction technology
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