HPLC法测定肝切除患者体内异甘草酸镁的血药浓度 点击下载
| 论文标题： | HPLC法测定肝切除患者体内异甘草酸镁的血药浓度 |
| 英文标题： | |
| 中文摘要： | 目的：建立测定肝切除患者体内门静脉血和外周静脉血中异甘草酸镁浓度的方法，为进一步验证和评估异甘草酸镁的药动学特征及临床合理、安全用药提供参考。方法：选择我院2014年10月－2015年3月施行肝部分切除术的患者31例，于手术开始时给予静脉应用异甘草酸镁，用药后1 h抽取患者门静脉血和外周静脉血，采用高效液相色谱-紫外检测法测定患者的血药浓度。结果：异甘草酸镁的保留时间为4.5 min，峰形良好，血浆内源峰对测定无干扰；异甘草酸镁血药浓度在0.55～55.00 mg/L范围内线性关系良好；最低定量浓度为0.55 mg/L。提取回收率为84.7％～87.1％，方法回收率为101.2％～105.4％，日内、日间精密度的RSD均＜6％；肝切除患者应用异甘草酸镁1 h后门静脉血药浓度明显高于外周静脉血药浓度（接近2倍），而且该浓度不受肝脏原发疾病和基础疾病（如肝硬化）的影响。结论：该测定方法具有操作简单、提取回收率高、准确度和灵敏度高等优点，能满足药动学研究的需要；肝切除患者术中应用异甘草酸镁后，在门静脉血中有较高的血药浓度，有利于其发挥保护肝细胞及改善肝功能的作用，适于在肝脏围术期使用。 |
| 英文摘要： | OBJECTIVE： To establish the method for the determination of plasma concentration of magnesium isoglycyrrhizinate in portal vein and peripheral venous blood of patients underwent liver resection， to further validate and evaluate pharmacokinetic characteristics， rational and safe use of drugs in the clinic. METHODS： 31 patients underwent liver resection in our hospital during Oct. 2014-Mar. 2015 were given magnesium isoglycyrrhizinate intravenously at the beginning of surgery. Portal vein and peripheral venous blood of patients were drawn at 1 hour after drug use， and HPLC-UV detection method was used to determine the plasma concentration of drug. RESULTS： The retention time of isoglycyrrhizinate magnesium was 4.5 min， which showed a good peak shape， and was not interfered with the determination by plasma endogenous peak. The plasma concentration ranged from 0.55 to 55.00 mg/L. The minimum quantitative concentration was 0.55 mg/L. The extraction recoveries were 84.7％-87.1％， and method recoveries were 101.2％-105.4％， and RSDs of intra-day and inter-day were less than 6 ％. Plasma concentration of magnesium isoglycyrrhizinate in portal vein blood was significantly higher than in peripheral vein blood of patients underwent liver resection （close to 2 times）； and plasma concentration was not affected by primary liver diseases and underlying diseases such as cirrhosis. CONCLUSIONS： The method is simple and has high recovery rate of extraction， high accuracy and high sensitivity. It can meet the needs of pharmacokinetic study. After the application of magnesium isoglycyrrhizinate during liver resection， there is higher blood concentration of magnesium isoglycyrrhizinate in portal vein， which is beneficial to protect liver cells and improve liver function. It is suitable during perioperative period of liver. |
| 期刊： | 2016年第27卷第17期 |
| 作者： | 张宪祥，高源，卢云1，张炳远，吴力群 |
| 英文作者： | ZHANG Xianxiang1，GAO Yuan1，LU Yun1，ZHANG Bingyuan2，WU Liqun |
| 关键字： | 异甘草酸镁；血药浓度；高效液相色谱法；药动学 |
| KEYWORDS： | Magnesium isoglycyrrhizinate； Plasma concentration； HPLC； Pharmacokinetics |
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| 文件大小： | 619.60Kb |
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