Man-PEI25k纳米复合物在微针作用下的透皮性能研究 点击下载
| 论文标题： | Man-PEI25k纳米复合物在微针作用下的透皮性能研究 |
| 英文标题： | |
| 中文摘要： | 目的：研究高分子质量聚乙烯亚胺（PEI25k）的甘露糖衍生物（Man-PEI25k）的纳米复合物在微针作用下的透皮性能。方法：以荧光染料水溶性羧基CdSe/ZnS量子点（QD）为模型药物，与PEI25k、Man-PEI25k溶液通过静电吸附形成PEI25k/QD和不同嫁接率（1 ∶ 3、1 ∶ 6）的Man-PEI25k/QD纳米复合物，同时以游离的QD作为对照，通过共聚焦显微镜观察微针作用下QD在皮肤活性表皮层和真皮层中的分布情况；用荧光分光光度计测定微针作用下PEI25k/QD和Man-PEI25k/QD纳米复合物在皮肤活性表皮层和真皮层中QD的累积滞留量。结果：微针作用于在体皮肤，Man-PEI25k/QD纳米复合物在活性表皮层和真皮层的量明显高于PEI25k/QD纳米复合物；Man-PEI25k/QD （1 ∶ 6） 纳米复合物在活性表皮层和真皮层的量明显高于Man-PEI25k/QD（1 ∶ 3） 纳米复合物。体外透皮试验显示，微针能明显提高纳米复合物在活性表皮层和真皮层中的滞留量；微针作用48 h后，Man-PEI25k/QD纳米复合物在活性表皮层和真皮层的滞留量约比PEI25k/QD增加了2、1.5倍，二者差异有统计学意义（P＜0.01）。结论：微针可提高Man-PEI25k/QD纳米复合物在活性表皮层的透皮性能。 |
| 英文摘要： | OBJECTIVE： To study the percutaneous properties of Man-PEI25k nanocomplex under the treatment of microneedles. METHODS： Using fluorescent dye water-soluble carboxyl CdSe/ZnS quantum dot （QD） as model drug， Man-PEI25k/QD and Man-PEI25k/QD nanocomplex with different grafting rates （1 ∶ 3， 1 ∶ 6） were formed through electrostatic adherence with PEI25k and Man-PEI25k. The distribution of QD in the active epidermal layer and dermis of skin were observed by confocal microscopy after the treatment of microneedles， using free QD as control. The accumulative retention amounts of QD in the active epidermal layer and dermis of skin were determined by fluorescence spectrophotometer after PEI25k/QD and Man-PEI25k/QD nanocomplex treated with microneedles. RESULTS： The amounts of Man-PEI25k/QD nanocomplex in active epidermal layer and dermis were significantly higher than that of PEI25k/QD nanocomplex under the treatment of microneedles in vivo； the amounts of Man-PEI25k/QD （1 ∶ 6） nanocomplex in active epidermal layer and dermis of skin were significantly higher than that of Man-PEI25k/QD （1 ∶ 3） nanocomplex. In in vitro transdermal diffusion experiments， microneedles could increase the retention amounts of nanocomplex in active epidermal layer and dermis of skin significantly. The retention amounts of Man-PEI25k/QD nanocomplex in active epidermal layer were increased by 2 times of that of PEI25k/QD under the treatment of microneedles after 48 h； at the same time， in the dermis that was increased by 1.5 times， with statistical significance （P＜0.01）. CONCLUSIONS： Microneedles can improve the percutaneous properties of Man-PEI25k nanocomplex in active epidermal layer. |
| 期刊： | 2016年第27卷第22期 |
| 作者： | 许娇娇，杨云旭，徐蓓华，胡英 |
| 英文作者： | XU Jiaojiao，YANG Yunxu，XU Beihua，HU Ying |
| 关键字： | 高分子质量聚乙烯亚胺的甘露糖衍生物；纳米复合物；微针；透皮给药；水溶性羧基CdSe/ZnS量子点 |
| KEYWORDS： | Man-PEI25k； Nanocomplex；Microneedles；Transdermal drug delivery； Water-soluble carboxyl CdSe/ZnS quantum dot |
| 总下载数： | 81次 |
| 本日下载数： | 2次 |
| 本月下载数： | 81次 |
| 文件大小： | 619.60Kb |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！