HPLC法同时测定精制冠心颗粒中5种成分的含量 点击下载
论文标题: | HPLC法同时测定精制冠心颗粒中5种成分的含量 |
英文标题: | |
中文摘要: | 目的:建立同时测定精制冠心颗粒中丹参素、丹酚酸B、原儿茶醛、芍药苷和阿魏酸含量的方法。方法:采用高效液相色谱法。色谱柱为 Zorbax Eclipse XDB-C18,流动相为乙腈-甲醇-0.5%磷酸(梯度洗脱),流速为1.0 ml/min,检测波长为280 nm(丹参素、原儿茶醛、丹酚酸B)、230 nm(芍药苷)、320 nm(阿魏酸),柱温为30 ℃,进样量为1.0 μl。结果:丹参素、原儿茶醛、丹酚酸B、芍药苷和阿魏酸检测质量浓度线性范围分别为1.19~478.34 μg/ml(r=0.999 9)、0.11~44.93 μg/ml(r=0.999 9)、7.49~995.20 μg/ml(r=0.999 7)、0.95~379.39 μg/ml(r=0.999 9)、0.01~3.12 μg/ml(r=0.999 5);定量限分别为1.91、0.36、150.00、2.74、0.10 ng,检测限分别为0.96、0.10、45.00、1.52、0.03 ng;精密度、稳定性、重复性试验的RSD<2%;加样回收率分别为98.06%~99.47%(RSD=0.52%,n=6)、98.01%~99.49%(RSD=0.70%,n=6)、98.44%~99.45%(RSD=0.37%,n=6)、96.94%~100.71%(RSD=1.27%,n=6)、95.44%~100.44%(RSD=1.90%,n=6)。结论:该方法操作简单、结果准确,适用于同时测定精制冠心颗粒中丹参素、原儿茶醛、丹酚酸B、芍药苷和阿魏酸的含量。 |
英文摘要: | OBJECTIVE: To establish a method for simultaneous determination of danshensu, salvianolic acid B, protocatechuic aldehyde, paeoniflorin and ferulic acid in Jingzhi guanxin granule. METHODS: HPLC was performed on the column of Zorbax Eclipse XDB-C18 with mobile phase of acetonitrile-methanol -0.5% H3PO4(gradient elution) at a flow rate of 1.0 ml/min, the detection wavelength was 280 nm (for danshensu, protocatechuic aldehyde, salvianolic acid B),230 nm (for ferulic acid) and 320 nm (for paeoniflorin), column temperature was 30 ℃,injection volume was 10 μl. RESULTS: The linear range was 1.19-478.34 μg/ml for danshensu(r=0.999 9), 0.11-44.93 μg/ml for protocatechuic aldehyde(r=0.999 9),7.49-995.20 μg/ml for salvianolic acid B(r=0.999 7), 0.95-379.39 μg/ml for paeoniflorin (r=0.999 9) and 0.01-3.12 μg/ml for ferulic acid (r=0.999 5); the limits of quantitation were 1.91 ng, 0.36 ng, 150.00 ng, 2.74 ng and 0.10 ng, limit of detection were 0.96 ng, 0.10 ng,45.00 ng,1.52 ng and 0.03 ng; RSDs of precision, stability and reproducibility tests were lower than 2%; recoveries were 98.06%-99.47% (RSD=0.52%,n=6), 98.01%-99.49% (RSD=0.70%,n=6), 98.44%-99.45% (RSD=0.37%,n=6), 96.94%-100.71% (RSD=1.27%,n=6) and 95.44%-100.44% (RSD=1.90%,n=6). CONCLUSIONS: The method is simple and accurate, and suitable for the simultaneous determination of danshensu, salvianolic acid B, protocatechuic aldehyde, paeoniflorin and ferulic acid in Jingzhi guanxin granule. |
期刊: | 2016年第27卷第30期 |
作者: | 姜晖,王玉,付连浩,张熙洁,刘晓红 |
英文作者: | JIANG Hui,WANG Yu,FU Lianhao,ZHANG Xijie,LIU Xiaohong |
关键字: | 含量测定;高效液相色谱法;丹参素;原儿茶醛;丹酚酸B;芍药苷;阿魏酸 |
KEYWORDS: | Content determination; HPLC; Danshensu; Protocatechuic aldehyde; Salvianolic acid B; Paeoniflorin; Ferulic acid |
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