RP-HPLC法同时测定半夏糖浆中乙酸和琥珀酸的含量 点击下载
论文标题: | RP-HPLC法同时测定半夏糖浆中乙酸和琥珀酸的含量 |
英文标题: | |
中文摘要: | 目的:建立同时测定半夏糖浆中乙酸和琥珀酸含量的方法。方法:采用反相高效液相色谱法。色谱柱为GL InterSustain-C18,流动相为0.03 mol/L磷酸二氢铵缓冲液(pH2.0)-甲醇(梯度洗脱),流速为0.8 ml/min,检测波长为210 nm,柱温为30 ℃,进样量为10 μl。结果:乙酸和琥珀酸检测质量浓度线性范围分别为0.020 98~0.209 8(r=0.999 9)、12.04~120.4 μg/ml(r=0.999 9);定量限分别为0.15、18.24 ng,检测限分别为0.045、5.53 ng;精密度、稳定性、重复性试验的RSD<2%;加样回收率分别为97.45%~101.68%(RSD=1.39%,n=9)、98.31%~101.08%(RSD=1.01%,n=9)。结论:该方法准确、快速,适用于同时测定半夏糖浆中乙酸和琥珀酸的含量。 |
英文摘要: | OBJECTIVE: To establish a method for the simultaneous determination of acetic acid and succinic acid in Banxia syrup. METHODS: RP-HPLC was performed on the column of GL InterSustain-C18 with mobile phase of 0.03 mol/L ammonium dihydrogen phosphate buffer solution (pH2.0)-methanol (gradient elution) at a flow rate of 0.8 ml/min, the detection wavelength was 210 nm, column temperature was 30 ℃, injection volume was 10 μl. RESULTS: The linear range was 0.020 98-0.209 8 μg/ml for acetic acid (0.999 9)and 12.04-120.4 μg/ml for succinic acid(r=0.999 9); limits of quantification were 0.15, 18.24 ng, limits of detection were 0.045, 5.53 ng; RSDs of precision, stability and reproducibility tests were lower than 2%; recoveries were 97.45%-101.68% (RSD=1.39%,n=9) and 98.31%-101.08% (RSD=1.01%,n=9). CONCLUSIONS: The method is accurate and rapid, and suitable for the simultaneous determination of acetic acid and succinic acid in Banxia syrup. |
期刊: | 2016年第27卷第30期 |
作者: | 何丹,杨晓梅,傅亚,秦少容,张景勍 |
英文作者: | HE Dan,YANG Xiaomei,FU Ya,QIN Shaorong,ZHANG Jingqing |
关键字: | 反相高效液相色谱法;半夏糖浆;乙酸;琥珀酸 |
KEYWORDS: | RP-HPLC; Banxia syrup; Acetic acid; Auccinic acid |
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