万古霉素壳聚糖缓释微球的制备及其体外释药特性和抑菌作用研究 点击下载
| 论文标题： | 万古霉素壳聚糖缓释微球的制备及其体外释药特性和抑菌作用研究 |
| 英文标题： | |
| 中文摘要： | 目的：制备万古霉素壳聚糖缓释微球，考察其体外释药特性和抑菌作用。方法：采用乳化交联法制备万古霉素壳聚糖缓释微球，观察微球的粒径分布及形态；应用紫外分光光度法测定微球药物含量，计算载药量及包封率；通过体外释放试验，比较其与盐酸万古霉素24 h内的体外释药情况，考察其7 d内的累积释放度（Q）；通过体外抑菌试验观察其作用20 d对金黄色葡萄球菌抑菌圈直径的影响。 结果：所制万古霉素壳聚糖缓释微球呈圆球形，表面圆整，无明显凹凸和孔隙，平均粒径为（165.6±19.7） μm，平均载药量为（32.6±3.2）％，平均包封率为（53.8±6.3）％，Q7 d为82.7％；其与盐酸万古霉素的Q2 h分别为18.6％、91.3％；其作用1 d时的抑菌圈直径＞3 cm，随其作用时间的延长，抑菌圈直径逐渐缩小。结论：成功制得万古霉素壳聚糖缓释微球，其体外缓释效果明显，且具有良好的体外抑菌作用。 |
| 英文摘要： | OBJECTIVE： To prepare vancomycin-loaded chitosan sustained-release microspheres and to study its drug release and antibacterial effects in vitro. METHODS： The emulsion cross-linking was used to prepare vancomycin-loaded chitosan sustained-release microspheres， and the particle size and morphology of microspheres were observed. The contents of vancomycin in microspheres was determined by UV spectrophotometry， and drug-loading amount of entrapment efficiency were calculated. Drug release of prepared microspheres and vancomycin hydrochloride within 24 h were compared by release trial in vitro， and then its accumulative release rate （Q） within 7 d was investigated. The effects of prepared microspheres on the diameter of inhibition zone to Staphylococcus aureus after treated for 20 d were observed through in vitro antibacterial test. RESULTS： The microspheres were spherical and complete round without obvious bump and pore. The average particle size of the microspheres was （165.6±19.7） μm； average drug-loading amount was （32.6±3.2）％； average encapsulation efficiency was （53.8±6.3）％； Q7 d was 82.7％. Q2 h of prepared microspheres and vancomycin hydrochloride were 18.6％ and 91.3％. The diameter of inhibition zone was higher than 3 cm after treated for 1 d， and then decreased gradually as time. CONCLUSIONS： Vancomycin-loaded chitosan sustained-release microspheres can be prepared successfully， and show obvious in vitro sustained-release effect and antibacterial effects. |
| 期刊： | 2016年第27卷第31期 |
| 作者： | 刘炜，刘菊，黄义新 |
| 英文作者： | LIU Wei，LIU Ju，HUANG Yixin |
| 关键字： | 万古霉素；壳聚糖；微球；体外释药；体外抑菌 |
| KEYWORDS： | Vancomycin； Chitosan； Microsphere； Release in vitro； Antibacterial effect in vitro |
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