基于非靶向代谢组学的兴安白芷不同部位差异代谢物分析 点击下载
| 论文标题： | 基于非靶向代谢组学的兴安白芷不同部位差异代谢物分析 |
| 英文标题： | |
| 中文摘要： | 目的 探讨兴安白芷根、茎、叶的代谢物差异，为白芷药用资源的开发和利用提供参考。方法采用超高效液相色谱-串联质谱非靶向代谢组学技术对兴安白芷根、茎、叶进行代谢物检测。采用多元统计分析方法筛选差异代谢物并进行聚类分析，进一步对差异代谢物进行京都基因与基因组百科全书（KEGG）通路富集分析。结果在兴安白芷根、茎、叶中共检测出769种代谢物，其中脂质类（14.31%）、生物碱类（13.91%）、氨基酸及其衍生物（13.65%）化合物占比较高，香豆素类化合物占比为3.25%。主成分分析、正交偏最小二乘判别分析及聚类分析结果一致表明，根、茎、叶的代谢物存在一定差异。茎与根的差异代谢物共205种（82种上调，123种下调），叶与根的差异代谢物共326种（247种上调，79种下调），叶与茎的差异代谢物共293种（263种上调，30种下调）。进一步对不同部位中香豆素类差异代谢物进行分析发现，（R）-氧化前胡素、4-羟基香豆素、4-甲基伞形酮乙酸酯等在叶中的相对含量较高。KEGG通路富集分析显示，差异代谢物主要富集于苯丙烷类物质生物合成、ABC转运蛋白等通路。结论兴安白芷根、茎、叶的代谢物存在较大差异。叶中香豆素类成分高度富集，其合成与转运可能与苯丙烷类生物合成、ABC转运蛋白通路的协同作用相关。 |
| 英文摘要： | OBJECTIVE To investigate the metabolic differences in roots， stems and leaves of Angelica dahurica （Fisch.ex Hoffm.） Benth. et Hook. f. ex Franch. et Savat. （shortened for A. dahurica ）， and to provide a basis for the development and utilization of its medicinal resources. METHODS UPLC-MS/MS untargeted metabolomics technology was employed to detect metabolites in roots， stems and leaves of A. dahurica . Multivariate statistical analysis was used to screen differential metabolites and perform cluster analysis. Furthermore， Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment analysis was conducted on these differential metabolites. RESULTS A total of 769 metabolites were identified in roots， stems and leaves of A. dahurica . Among t hem， lipids， alkaloids， amino acids and their derivatives accounted for the highest proportion， which accounted for 14.31%， 13.91% and 13.65%， respectively， and the coumarin compounds accounted for 3.25%. Results of principal component analysis， orthogonal partial least squares-discriminant analysis and cluster analysis indicated that there were certain differences in metabolites among the three tissues. A total of 205 differential metabolites were identified between stems and roots （82 up-regulated and 123 down-regulated）， 326 differential metabolites between leaves and roots （247 up-regulated and 79 down-regulated）， and 293 differential metabolites between the leaves and stems （263 up-regulated and 30 down-regulated）. Further analysis of the differential coumarin metabolites in different plant parts revealed that several compounds， including （ R ）-oxypeucedanin， 4-hydroxycoumarin， 4-methylumbelliferyl acetate， exhibited relatively high contents in the leaves. KEGG pathway enrichment analysis indicated that differential metabolites predominantly accumulated in the phenylpropanoid biosynthesis and ABC transporter pathways. CONCLUSIONS There are significant differences in metabolites among roots， stems and leaves of A. dahurica . Coumarin compounds are highly enriched in the leaves， and their synthesis and transport may be related to the synergistic effect of the phenylpropanoid biosynthesis pathway and the ABC transporter pathway. |
| 期刊： | 2026年第37卷第09期 |
| 作者： | 王星；周紫珊；田恩伟 |
| 英文作者： | WANG Xing，ZHOU Zishan，TIAN Enwei |
| 关键字： | 兴安白芷；非药用部位；非靶向代谢组学；差异代谢物；资源开发；UPLC-MS/MS |
| KEYWORDS： | Angelica dahurica （Fisch.ex Hoffm.） Benth. et Hook. f. ex Franch. et Savat.； non-medicinal parts； untargeted |
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