龙眼叶活性成分配伍改善2型糖尿病大鼠糖脂代谢紊乱的机制 点击下载
| 论文标题： | 龙眼叶活性成分配伍改善2型糖尿病大鼠糖脂代谢紊乱的机制 |
| 英文标题： | |
| 中文摘要： | 目的 探讨龙眼叶活性成分（槲皮素、槲皮苷、山柰酚，质量比为2∶9∶3）配伍（简称CDL）改善2型糖尿病（T2DM）大鼠糖脂代谢紊乱的作用机制。方法将SD大鼠随机分为空白对照组、模型组、盐酸二甲双胍组（100mg/kg）和CDL高、中、低剂量组（280、140、75mg/kg），每组10只。空白对照组大鼠喂养维持饲料，其余各组大鼠喂养高糖高脂饲料并腹腔注射链脲佐菌素以复制T2DM模型。造模成功后，各给药组大鼠灌胃相应药液，空白对照组和模型组大鼠灌胃等体积纯水，每天1次，连续4周。每周固定时间检测大鼠空腹血糖（FBG），绘制口服葡萄糖耐量试验（OGTT）曲线和腹腔注射胰岛素耐量试验（IPITT）曲线，并计算曲线下面积（AUC）；检测大鼠胰岛功能指标[空腹胰岛素（FINS）、稳态模型评估胰岛素抵抗指数（HOMA-IR）和胰岛素敏感指数（ISI）]和血脂指标[总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）]以及肝糖原含量；观察大鼠肝脏和胰腺组织的病理学形态变化；检测大鼠肝脏组织中磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（Akt）信号通路相关蛋白和mRNA表达水平。结果与空白对照组比较，模型组大鼠FBG、IPITT曲线AUC、OGTT曲线AUC、HOMA-IR，FINS、TC、TG、LDL-C水平，肝脏组织中磷酸酶张力蛋白同源物、叉头框蛋白O1、糖原合成酶激酶3β的蛋白和mRNA表达水平均显著增加/升高（P＜0.05）；ISI、HDL-C水平、肝糖原含量，肝脏组织中PI3K、胰岛素受体底物1、Akt的蛋白和mRNA表达水平以及磷酸化Akt蛋白表达水平均显著降低（P＜0.05）；肝细胞排列不规则，胰腺小叶整体结构紊乱，且均出现大量炎症细胞浸润。与模型组比较，CDL高剂量组大鼠上述大部分定量指标均显著逆转（P＜0.05）；肝脏和胰腺病变明显减轻。结论CDL可调节T2DM大鼠的糖脂代谢紊乱，提高胰岛素敏感性，改善胰岛素抵抗，其作用机制可能与激活PI3K/Akt信号通路有关。 |
| 英文摘要： | OBJECTIVE To explore the mechanism of the active ingredient compatibility（quercetin， quercitrin and kaempferol at a mass ratio of 2∶9∶3）of Dimocarpus longan Lour. leaves（abbreviated as CDL） on ameliorating glucose and lipid metabolism disorders in type 2 diabetes mellitus （T2DM） rats. METHODS SD rats were randomly divided into blank control group， model group， metformin hydrochloride group （100 mg/kg）， and CDL high-， medium- and low-dose groups （280， 140， 75 mg/kg）， with 10 rats in each group. Rats in the blank control group were fed with standard chow， while rats in the other groups were given high-sugar and high-fat diet combined with intraperitoneal injection of streptozotocin to establish the T2DM rat model. After successful modeling， rats in each administration group were given corresponding drug solution， and rats in the blank control group and model group were intragastrically administered with equal volume of pure water， once a day， for consecutive 4 weeks. Fasting blood glucose （FBG） was detected at fixed time every week. The curves of oral glucose tolerance test （OGTT） and intraperitoneal insulin tolerance test （IPITT） were plotted， and the area under curve （AUC） was calculated. The pancreatic islet function indexes [fasting insulin （FINS）， homeostasis model assessment of insulin resistance （HOMA-IR）， insulin sensitivity index （ISI）]，blood lipid indexes [total cholesterol （TC）， triglyceride （TG）， low-density lipoprotein cholesterol （LDL-C）， high-density lipoprotein cholesterol （HDL-C）] and hepatic glycogen content were determined. The pathological morphological changes of liver and pancreatic tissues were observed. The protein and mRNA expression levels of molecules related to phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） signaling pathway in liver tissues were detected. RESULTS Compared with the blank control group， the FBG， AUC of IPITT curve， AUC of OGTT curve， HOMA-IR， the levels of FINS， TC， TG and LDL-C， as well as the protein and mRNA expression of phosphatase and tensin homolog， forkhead box protein O1 and glycogen synthase kinase-3β in liver tissues were significantly increased in the model group （ P ＜0.05）. ISI， the levels of HDL-C and hepatic glycogen content， along with the protein and mRNA expression of PI3K， insulin receptor substrate-1， Akt and protein expression of phosphorylated Akt in liver tissues were markedly decreased （ P ＜0.05）. In model group rats， the arrangement of hepatocytes was irregular， the overall structure of pancreatic lobules was disordered， and a large number of inflammatory cell infiltration was observed. Compared with the model group， most of the above quantitative indexes were significantly reversed in the CDL high-dose group （ P ＜0.05）， and the pathological lesions of liver and pancreas were obviously alleviated. CONCLUSIONS CDL can regulate glucose and lipid metabolism disorders， elevate insulin sensitivity and relieve insulin resistance in T2DM rats. Its mechanism may be related to the activation of the PI3K/Akt signaling pathway. |
| 期刊： | 2026年第37卷第13期 |
| 作者： | 梁炎丽；安施佳；李丰盛；麦嘉妮；霍安琪；韦佳丽；张泽娟；覃淑艳；黄文清；梁洁 |
| 英文作者： | LIANG Yanli，AN Shijia， LI Fengsheng，MAI Jiani，HUO Anqi，WEI Jiali，ZHANG Zejuan，QIN Shuyan，HUANG Wenqing，LIANG Jie |
| 关键字： | 2型糖尿病; 龙眼叶; 活性成分配伍; 槲皮素; 槲皮苷; 山柰酚; PI3K/Akt信号通路 |
| KEYWORDS： | type 2 diabetes mellitus; Dimocarpus longan Lour. leaves; active ingredient compatibility; quercetin; quercitrin; kaempferol; PI3K/Akt signaling pathway |
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