基于标准煎液的红芪金颗粒提取工艺研究 点击下载
| 论文标题： | 基于标准煎液的红芪金颗粒提取工艺研究 |
| 英文标题： | |
| 中文摘要： | 目的 以红芪金方标准煎液为参照，进行红芪金颗粒的提取工艺研究。方法制备15批红芪金方标准煎液，建立其对照特征图谱并计算相似度，测定其平均出膏率及毛蕊异黄酮苷、红景天苷、姜黄素、大黄素的平均转移率；以浸泡时间、加水量、煎煮时间为因素，以提取液出膏率、指标成分转移率及与对照特征图谱的相似度为指标，采用正交实验结合主成分分析优化红芪金颗粒提取工艺，并进行验证。结果15批红芪金方标准煎液的平均出膏率为32.55%，毛蕊异黄酮苷、红景天苷、姜黄素、大黄素的平均转移率分别为70.68%、74.35%、2.16%、4.62%。红芪金颗粒最佳提取工艺为：提取2次，第1次加8倍量水，提取60min；第2次加6.5倍量水，提取45min。3次验证实验的平均出膏率为33.01%；上述4种指标成分的平均转移率分别为79.51%、75.54%、1.84%、4.30%，RSD均小于2%；与对照特征图谱的相似度均大于0.990。结论优选的红芪金颗粒提取工艺稳定，且提取液与红芪金方标准煎液的物质基础相一致。 |
| 英文摘要： | OBJECTIVE To study the extraction process of Hongqijin granules with the standard decoction of Hongqijin formula as the reference. METHODS Fifteen batches of Hongqijin formula standard decoction were prepared. The reference characteristic chromatogram was established， and the chromatographic similarity was calculated. The average extraction yield and average transfer rates of calycosin-7-glucoside， salidroside， curcumin， and emodin of the 15 batches of standard decoction were determined. Taking soaking time， water addition amount， and decoction time as influencing factors， and extraction yield， transfer rates of marker components， as well as chromatographic similarity with the reference characteristic chromatogram as evaluation indexes， orthogonal test combined with principal component analysis was adopted to optimize the extraction process of Hongqijin granules， followed by process verification. RESULTS The average extraction yield of 15 batches of Hongqijin formula standard decoction was 32.55%. The average transfer rates of calycosin-7-glucoside， salidroside， curcumin， and emodin were 70.68%， 74.35%， 2.16%， and 4.62%， respectively. The optimal extraction process of Hongqijin granules was confirmed as follows： extraction was carried out twice； the first extraction used 8-fold water for 60 min， and the second extraction adopted 6.5-fold water for 45 min. In three validation tests， the average extraction yield was 33.01%. The average transfer rates of the four marker components were 79.51%， 75.54%， 1.84%， and 4.30%， with all RSD values less than 2%. The chromatographic similarities between the extracting solution and reference characteristic chromatogram were all higher than 0.990. CONCLUSIONS The optimized extraction process of Hongqijin granules exhibits good stability， and the material basis of the extracting solution is consistent with that of Hongqijin formula standard decoction. |
| 期刊： | 2026年第37卷第15期 |
| 作者： | 覃翎瑞；吴旭彤；孙奕成；邹恺平；田立元；谭喜莹；薛静娴；赵晨光；刘顺 |
| 英文作者： | QIN Lingrui，WU Xutong， SUN Yicheng，ZOU Kaiping，TIAN Liyuan，TAN Xiying，XUE Jingxian，ZHAO Chenguang，LIU Shun |
| 关键字： | 红芪金颗粒；标准煎液；提取工艺；特征图谱；正交实验；主成分分析；物质基础 |
| KEYWORDS： | Hongqijin granules； standard decoction； |
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| 文件大小： | 619.60Kb |
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