益气养阴活血方对IgA肾病大鼠肾损伤的改善作用及其与NEAT1/RACK1/NLRP3通路的关系 点击下载
| 论文标题： | 益气养阴活血方对IgA肾病大鼠肾损伤的改善作用及其与NEAT1/RACK1/NLRP3通路的关系 |
| 英文标题： | |
| 中文摘要： | 目的 研究益气养阴活血方对免疫球蛋白A肾病（IgAN）大鼠肾损伤的改善作用，并基于核富集转录本1（NEAT1）/活化的蛋白激酶C受体1（RACK1）/NOD样受体热蛋白结构域相关蛋白3（NLRP3）通路探讨其可能的分子机制。方法将SD大鼠分为空白组与造模组，造模组大鼠采用“牛血清白蛋白+四氯化碳+脂多糖”联合法诱导IgAN模型。将造模成功的大鼠随机分为模型组、醋酸泼尼松组（6.25mg/kg）和益气养阴活血方低、高剂量组（11、22g/kg）。各组大鼠灌胃相应的药物或生理盐水，每天1次，连续6周。末次给药后检测大鼠尿红细胞计数、24h尿蛋白（24h-UTP）以及血清中肌酐（Cr）、尿素氮（BUN）、白蛋白（ALB）、丙氨酸氨基转移酶（ALT）、白细胞介素1β（IL-1β）水平；观察大鼠肾组织病理学形态变化以及肾小球系膜区免疫球蛋白A（IgA）沉积情况；检测大鼠肾组织中NEAT1mRNA及RACK1、NLRP3mRNA和蛋白表达水平。结果与空白组比较，模型组大鼠肾小球系膜区基质增生伴炎症细胞浸润，肾小管广泛扩张伴局灶性萎缩；24h-UTP、尿红细胞计数，血清中IL-1β、Cr、BUN、ALT水平，肾小球系膜区IgA的平均荧光强度，肾组织中NEAT1mRNA以及RACK1、NLRP3mRNA和蛋白表达水平均显著升高（P＜0.05）；血清中ALB水平显著降低（P＜0.05）。与模型组比较，益气养阴活血方低、高剂量组大鼠肾小球结构基本正常，肾小管损伤明显减轻；上述定量指标水平均显著逆转（P＜0.05）。结论益气养阴活血方可减轻炎症反应、改善IgAN大鼠肾损伤，其作用机制可能与抑制NEAT1/RACK1/NLRP3通路活性有关。 |
| 英文摘要： | OBJECTIVE To investigate the improving effect of Yiqi yangyin huoxue formula on renal injury in immunoglobulin A nephropathy （IgAN） rats， and explore its potential molecular mechanism based on nuclear-enriched abundant transcript 1 （NEAT1）/receptor of activated C kinase 1 （RACK1）/NOD-like receptor family pyrin domain containing 3 （NLRP3） pathway.METHODS SD rats were divided into blank group and model-establishing group. Rats in the model-establishing group were induced to establish IgAN model by combined treatment of bovine serum albumin， carbon tetrachloride and lipopolysaccharide. Rats with successful model establishment were randomly divided into model group， prednisone acetate group （6.25 mg/kg）， low-dose and high-dose Yiqi yangyin huoxue formula groups （11， 22 g/kg）. Each group was intragastrically administered corresponding drugs or normal saline once a day for 6 consecutive weeks. After the last administration， urinary red blood cell count， 24-hour urinary total protein （24 h-UTP）， serum creatinine （Cr）， blood urea nitrogen （BUN）， albumin （ALB）， alanine amino-transferase （ALT） and interleukin-1β （IL-1β） levels were detected. Pathomorphological changes of renal tissues and immunoglobulin A （IgA） deposition in glomerular mesangial region were observed. The mRNA expression of NEAT1， as well as mRNA and protein expression levels of RACK1 and NLRP3 in renal tissues were determined.RESULTS Compared with the blank group， the model group presented glomerular mesangial matrix hyperplasia accompanied by inflammatory cell infiltration， and extensive renal tubular dilation with focal atrophy. The levels of 24 h-UTP， urinary red blood cell count， serum IL-1β， Cr， BUN， ALT， mean fluorescence intensity of IgA deposition in glomerular mesangial area， mRNA expression level of NEAT1， mRNA and protein expression levels of RACK1 and NLRP3 in renal tissues were significantly increased （ P ＜0.05）， while serum ALB level was significantly decreased （ P ＜0.05）. Compared with the model group， the glomerular structure was basically restored and renal tubular injury was obviously alleviated in low-dose and high-dose Yiqi yangyin huoxue formula groups； all the above quantitative indicators were significantly reversed （ P ＜0.05）.CONCLUSIONS Yiqi yangyin huoxue formula can mitigate the inflammatory response and ameliorate renal injury in IgAN rats， and its mechanism may be related to the inhibition of NEAT1/RACK1/NLRP3 pathway activity. |
| 期刊： | 2026年第37卷第17期 |
| 作者： | 郏溪栋；宋纯东；王旭；陶伊璠 |
| 英文作者： | JIA Xidong，SONG Chundong，WANG Xu，TAO Yifan |
| 关键字： | 益气养阴活血方；免疫球蛋白A肾病；炎症反应；肾损伤；NEAT1/RACK1/NLRP3通路 |
| KEYWORDS： | immunoglobulin A nephropathy;inflammatory response;renal injury;NEAT1/RACK1/NLRP3 pathway |
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