杏花中绿原酸提取工艺优化及不同产地和品种杏花中绿原酸的含量比较 点击下载
| 论文标题： | 杏花中绿原酸提取工艺优化及不同产地和品种杏花中绿原酸的含量比较 |
| 英文标题： | |
| 中文摘要： | 目的：优化杏花中绿原酸的提取工艺，比较不同产地、不同品种杏花中绿原酸的含量。方法：采用高效液相色谱法测定杏花中绿原酸含量；以提取溶剂乙醇的体积分数、料液比、超声提取次数和超声提取时间为因素，以绿原酸含量为指标，设计单因素和正交试验优化提取工艺，并进行验证试验。采用优化后提取工艺提取并比较7个产地杏花和3个产地山杏花中绿原酸的含量。结果：最优提取工艺为加12倍量的75％乙醇，超声提取2次，每次30 min。在此条件下，杏花绿原酸含量可达77.38 mg/g（RSD＝0.58％，n＝3）；山杏花、杏花中绿原酸含量分别为77.38～83.33 、63.12～70.22 mg/g。结论：建立的提取工艺合理、稳定、可行；山杏花中绿原酸含量较杏花高，同品种、不同产地的杏花中绿原酸含量差异不大。 |
| 英文摘要： | OBJECTIVE： To optimize the extraction technology of chlorogenic acid in Prunus armeniaca flos， and compare the contents in P. armeniaca flos from different origins and varieties. METHODS： HPLC was conducted to determine the content of chlorogenic acid in P. armeniaca flos； using the volume fraction of ethanol， the ratio of material to liquid， ultrasonic extraction times and time as factor， the content of chlorogenic acid as index， single factor and orthogonal test were designed to optimize the extraction technology， and verification tests were carried out. The optimized extraction technology was used to extract and compare the contents of chlorogenic acid in Armeniaca sibirica from 7 origins of P. armeniaca flos and 3 origins of Armeniaca sibirica flos. RESULTS： The optimized extraction technology was extracting twice with 12-fold 75％ ethanol， 30 min each time. Under the conditions， the content of chlorogenic acid can reach 77.38 mg/g （RSD＝0.58％， n＝3）； the contents of chlorogenic acid in A. sibirica flos and P. armeniaca flos were 77.38-83.33 mg/g and 63.12-70.22 mg/g， respectively. CONCLUSIONS： The established extraction technology is reasonable， stable and feasible. The contents of chlorogenic acid in A. sibirica are higher than that in P. armeniaca flos； the contents have no obvious differences in the same variety of A. sibirica from different origins. |
| 期刊： | 2017年第28卷第7期 |
| 作者： | 罗慧玉，闫伟伟，徐鹏，曾春萍 |
| 英文作者： | LUO Huiyu，YAN Weiwei，XU Peng，ZENG Chunping |
| 关键字： | 杏花；绿原酸；正交试验；提取工艺；优化；产地；品种 |
| KEYWORDS： | Prunus armeniaca flos； Chlorogenic acid； Orthogonal test； Extraction technology； Optimization； Origin； Variety |
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| 文件大小： | 619.60Kb |
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