UPLC法同时测定缬沙坦氢氯噻嗪片中缬沙坦和氢氯噻嗪的含量 点击下载
论文标题: UPLC法同时测定缬沙坦氢氯噻嗪片中缬沙坦和氢氯噻嗪的含量
英文标题:
中文摘要: 目的:建立同时测定缬沙坦氢氯噻嗪片中缬沙坦和氢氯噻嗪含量的方法。方法:采用超高效液相色谱法。色谱柱为Phenomenex C18,流动相为[0.1%磷酸溶液-乙腈(95 ∶ 5,V/V)]-[0.1%磷酸溶液-乙腈(5 ∶ 95,V/V)](梯度洗脱),流速为0.25 mL/min,检测波长为272 nm,柱温为35 ℃,进样量为1.5 μL。结果:缬沙坦和氢氯噻嗪检测质量浓度线性范围分别为8.1~324.2 μg/mL(r=0.999 9)、1.2~50.1 μg/mL (r=0.999 9);定量限分别为0.24、0.04 ng,检测限分别为0.06、0.01 ng;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为97.69%~100.35%(RSD=1.03%,n=9)、98.27%~100.60%(RSD=0.83%,n=9)。结论:该方法操作简单、快速,结果准确,可用于缬沙坦氢氯噻嗪片中缬沙坦和氢氯噻嗪含量的同时测定。
英文摘要: OBJECTIVE: To establish a method for simultaneous determination of valsartan and hydrochlorothiazide in Valsartan hydrochlorothiazide tablets. METHODS: UPLC was adopted. The determination was performed on Phenomenex C18 column with mobile phase consisted of [0.1% phosphoric acid solution-acetonitrile (95 ∶ 5,V/V)]-[0.1% phosphoric acid solution-acetonitrile (5 ∶ 95,V/V)] (gradient elution) at the flow rate of 0.25 mL/min. The detection wavelength was set at 272 nm, and the column temperature was 35 ℃. The sample size was 1.5 μL. RESULTS: The linear range were 8.1-324.2 μg/mL for valsartan (r=0.999 9) and 1.2-50.1 μg/mL for hydrochlorothiazide (r=0.999 9). The limits of quantitation were 0.24, 0.04 ng, and the limits of detection were 0.06, 0.01 ng. RSDs of precision, stability and reproducibility tests were less than 2.0%; recoveries were 97.69%-100.35% for valsartan (RSD=1.03%,n=9) and 98.27%-100.60% for hydrochlorothiazide (RSD=0.83%,n=9). CONCLUSIONS: The method is simple, rapid and accurate, and can be used for the simultaneous determination of valsartan and hydrochlorothiazide in Valsartan hydrochlorothiazide tablets.
期刊: 2017年第28卷第15期
作者: 纪宏,刘晶,王威,祁进,刘清华
英文作者: JI Hong,LIU Jing,WANG Wei,QI Jin,LIU Qinghua
关键字: 超高效液相色谱法;缬沙坦氢氯噻嗪片;缬沙坦;氢氯噻嗪;含量
KEYWORDS: UPLC; Valsartan hydrochlorothiazide tablet; Valsartan; Hydrochlorothiazide; Content
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