改进HPLC法测定酮洛芬肠溶胶囊中酮洛芬的含量 点击下载
论文标题: 改进HPLC法测定酮洛芬肠溶胶囊中酮洛芬的含量
英文标题:
中文摘要: 目的:改进高效液相色谱法测定酮洛芬肠溶胶囊中酮洛芬的含量。方法:采用高效液相色谱法。色谱柱为Chiralpak IC ,流动相为正己烷(含0.1%三氟乙酸)-异丙醇(90 ∶ 10,V/V),流速为0.8 mL/min,检测波长为268 nm,柱温为25 ℃,进样量为10 μL。结果:酮洛芬检测质量浓度线性范围为0.025~0.5 mg/mL(r=0.999 8),定量限为1.0 mg/L,检测限为0.2 mg/L;精密度、稳定性、重复性试验的RSD<2%;加样回收率为96.36%~100.32%(RSD=1.87%,n=6)。结论:该方法操作简便,准确快速,适用于测定酮洛芬肠溶胶囊中酮洛芬的含量。
英文摘要: OBJECTIVE: To improve HPLC for content determination of ketoprofen in Ketoprofen enteric-coated capsules. METHODS: HPLC method was adopted. The determination was performed on Chiralpak IC column with mobile phase consisted of n-hexane  (0.1% TFA)-isopropanol (90 ∶ 10,V/V) at a flow rate of 0.8 mL/min. The detection wavelength was set at 268 nm, and column temperature was 25 ℃. The sample size was 10 μL. RESULTS: The linear range of ketoprofen were 0.025-0.5 mg/mL(r=0.999 8). The limit of quantitation was 1.0 mg/L, and limit of detection was 0.2 mg/L. RSDs of precision, stability and reproducibility tests were lower than 2%; recoveries were 96.36%-100.32% (RSD=1.87%,n=6). CONCLUSIONS: The method is simple, accurate and rapid, and can be used for the content determination of ketoprofen in Ketoprofen enteric-coated capsules.
期刊: 2017年第28卷第18期
作者: 郑旭霞,周宇雪,刘培建
英文作者: ZHENG Xuxia,ZHOU Yuxue,LIU Peijian
关键字: 酮洛芬肠溶胶囊;酮洛芬;含量;高效液相色谱法
KEYWORDS: Ketoprofen enteric-coated capsules; Ketoprofen; Content; HPLC
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