UPLC法同时测定连参通淋片中5种黄连生物碱类成分的含量 点击下载
论文标题: UPLC法同时测定连参通淋片中5种黄连生物碱类成分的含量
英文标题:
中文摘要: 目的:建立同时测定连参通淋片中表小檗碱、盐酸药根碱、黄连碱、盐酸巴马汀和盐酸小檗碱含量的方法。 方法:采用超高效液相色谱法。色谱柱为Thermo Scientific Syncronis C18,流动相为乙腈-0.1%磷酸溶液(加三乙胺调pH至3.0)(30 ∶ 70,V/V),流速为0.3 mL/min,检测波长为345 nm,柱温为 25 ℃,进样量为2 μL。结果:表小檗碱、盐酸药根碱、黄连碱、盐酸巴马汀和盐酸小檗碱检测进样量线性范围分别为4.08~163 ng(r=0.999 9)、5.72~229 ng(r=0.999 8)、5.67~227 ng(r=0.999 9)、6.78~271 ng(r=0.999 6)、13.2~526 ng(r=0.999 9);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为96.83%~103.92%(RSD=2.2%,n=9)、97.62%~104.85%(RSD=2.9%,n=9)、99.44%~104.18%(RSD=1.8%,n=9)、98.64%~104.42%(RSD=1.8%,n=9)、98.24%~102.40%(RSD=1.5%,n=9)。结论:该方法简便、灵敏、快速、准确,适用于连参通淋片中5种黄连生物碱类成分含量的同时测定。
英文摘要: OBJECTIVE: To establish the method for simultaneous determination of epiberberine, jateorhizine hydrochloride, coptisine, palmatine hydrochloride and berberine hydrochloride in Lianshen tonglin tablets. METHODS: HPLC method was adopted. The determination was performed on Thermo Scientific Syncronis C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution(pH adjusted to 3.0 with triethylamine)(30 ∶ 70,V/V) at the flow rate of 0.3 mL/min. The detection wavelength was set at 345 nm, and column temperature was 25 ℃. The sample size was 2 μL. RESULTS: The linear ranges were 4.08-163 ng for epiberberine(r=0.999 9), 5.72-229 ng for jateorhizine hydrochloride (r=0.999 8), 5.67-227 ng for coptisine(r=0.999 9), 6.78-271 ng for palmatine hydrochloride(r=0.999 6),13.2-526 ng for berberine hydrochloride(r=0.999 9). RSDs of precision, stability and reproducibility tests were lower than 2.0%. The recoveries were 96.83%-103.92%(RSD=2.2%,n=9), 97.62%-104.85%(RSD=2.9%,n=9),99.44%-104.18%(RSD=1.8%,n=9), 98.64%-104.42%(RSD=1.8%,n=9),98.24%-102.40%(RSD=1.5%,n=9),respectively. CONCLUSIONS: The developed method is simple, sensitive, rapid and accurate. It is suitable for simultaneous determination of 5 coptis alkaloids in Lianshen tonglin tablets.
期刊: 2017年第28卷第33期
作者: 周亚楠,白洁,袁浩,刘永利
英文作者: ZHOU Yanan,BAI Jie,YUAN Hao,LIU Yongli
关键字: 超高效液相色谱法;黄连生物碱;连参通淋片;含量
KEYWORDS: UPLC; Coptis alkaloid;Lianshen tonglin tablets; Content
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