蝉蜕蛋白的提取工艺优化及体外抗氧化活性研究 点击下载
论文标题: 蝉蜕蛋白的提取工艺优化及体外抗氧化活性研究
英文标题:
中文摘要: 目的:优化蝉蜕蛋白的提取工艺,并考察其体外抗氧化活性,为蝉蜕蛋白的深入研究提供参考。方法:以蛋白提取量为响应值,在单因素试验基础上,采用Box-Behnken响应面法对超声波辅助提取蝉蜕蛋白的液料比、超声时间、提取次数等条件进行优化,并进行验证试验。以维生素C(VC)为阳性对照,以对1,1-二苯基-2-三硝基苯肼(DPPH)和2,2′ -连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)(ABTS)自由基的清除率为指标,评价蝉蜕蛋白的体外抗氧化活性。结果:蝉蜕蛋白的最佳提取条件为液料比28 ∶ 1(mL/g),超声时间65 min,提取2次。验证试验中,蝉蜕蛋白的平均提取量为65.45 mg/g(RSD=1.68%,n=3),与预测值的相对误差为5.48%。蝉蜕蛋白对ABTS和DPPH自由基均有一定的清除效果,当蝉蜕蛋白质量浓度为0.2 mg/mL时对ABTS自由基的清除率即达97.0%,其作用与VC相当;蝉蜕蛋白对DPPH自由基的清除能力稍弱,半数清除浓度为0.96 mg/mL,其作用不及VC。结论:采用Box-Behnken响应面法优化蝉蜕蛋白的提取条件准确、可靠;蝉蜕蛋白具有一定的体外抗氧化活性。
英文摘要: OBJECTIVE: To optimize extraction technology of protein from Cryptotympana pustulata and investigate its in vitro antioxidant activity, so as to provide reference for further research of protein from C. pustulata. METHODS: Using extraction amount of protein as response value, based on single factor test, Box-Behnken response surface methodology was used to optimize the ratio of liquid to material, ultraonic time and extraction times. Validation test was conducted. Using Vitamin C (VC) as positive control, in vitro antioxidant activity of protein from C. pustulata was evaluated by using scavenging rate of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′ -azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) (ABTS) free radical as index. RESULTS: The optimal extraction condition of protein from C. pustulata was as follows as the ratio of liquid to material 28 ∶ 1 (mL/g), ultrasonic time of 65 min, extracting for twice. In validation test, average extraction amount of protein from C. pustulata was 65.45 mg/g(RSD=1.68%,n=3), relative error of which to predicted value was 5.48%. The protein from C. pustulata showed strong scavenging effect on ABTS and DPPH free radicals. When the concentration of protein from C. slough was 0.2 mg/mL, and the scavenging rate of it to ABTS free radicals was 97%, the effect of which was similar to VC. The protein from C. pustulata showed weak scavenging ability to DPPH free radical, IC50 was 0.96 mg/mL, the effect of which was not as good as VC. CONCLUSIONS: The extraction technology of protein from C. pustulata optimized by Box-Behnken response surface methodology shows high accuracy and good reliability. The protein from C. pustulata shows certain antioxidant activity in vitro.
期刊: 2018年第29卷第7期
作者: 李涛,程雪娇,胡美变,刘玉杰,吴纯洁
英文作者: LI Tao,CHENG Xuejiao,HU Meibian,LIU Yujie,WU Chunjie
关键字: 蝉蜕蛋白;超声波辅助提取;工艺优化;Box-Behnken响应面法;体外抗氧化活性
KEYWORDS: Protein from Cryptotympana pustulata; Ultrasonic assisted extraction; Technology optimization; Box-Behnken response surface methodology; Antioxidant activity in vitro
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