HPLC法同时测定还少胶囊中4种成分的含量 点击下载
论文标题: HPLC法同时测定还少胶囊中4种成分的含量
英文标题:
中文摘要: 目的:建立同时测定还少胶囊中莫诺苷、马钱苷、松果菊苷和毛蕊花糖苷含量的方法。方法:采用高效液相色谱法。色谱柱为中谱红RD-C18,流动相为乙腈-0.1%甲酸水溶液(梯度洗脱),检测波长为240nm(莫诺苷、马钱苷)、330nm(松果菊苷、毛蕊花糖苷),流速为1.0mL/min,柱温为35℃,进样量为10μL。结果:莫诺苷、马钱苷、松果菊苷、毛蕊花糖苷检测质量浓度的线性范围分别为5.29~105.80、4.49~89.88、16.26~325.25、16.31~326.25μg/mL(r均为0.9999);精密度、稳定性(24h)、重复性、耐用性试验的RSD均小于2.0%;加样回收率分别为94.34%~96.23%(RSD=0.81%,n=6)、97.04%~98.89%(RSD=0.73%,n=6)、96.23%~98.08%(RSD=0.82%,n=6)、95.40%~98.47%(RSD=1.23%,n=6)。11批还少胶囊中上述4种成分的含量分别为0.190~0.704、0.439~0.857、2.723~4.475、0.589~1.035mg/g。结论:所建方法专属性强、精密度好,可用于还少胶囊中4种成分的含量测定。
英文摘要: OBJECTIVE:To estab lish a me thod for simultaneous determination of morroniside ,loganin,echinacoside and acteoside in Huanshao capsules. METHODS :HPLC method was adopted. The determination was performed on Zhongpuhong RD-C18 column with mobile phase consisted of acetonitrile- 0.1% formic acid solution (gradient elution )at the flow rate of 1.0 mL/min. The detection wavelength was set at 240 nm (morroniside,loganin) and 330 nm (echinacoside,acteoside). The column temperature was set at 35 ℃,and sample size was 10 μL. RESULTS:The linear range were 5.29-105.80 μg/mL for morroniside, 4.49-89.88 mg/L for loganin ,16.26-325.25 mg/L for echinacoside and 16.31-326.25 mg/L for acteoside ,r values were 0.999 9. RSDs of precision ,stability (24 h),reproducibility and durability tests were all lower than 2.0% . The recoveries were 94.34% -96.23%(RSD=0.81% ,n=6),97.04% -98.89%(RSD=0.73% ,n=6),96.23% -98.08%(RSD=0.82% ,n=6), 95.40%-98.47%(RSD=1.23%,n=6),respectively. The contents of above 4 components in 11 batches of Huanshao Capsules were 0.190-0.704,0.439-0.857,2.723-4.475 and 0.589-1.035 mg/g,respectively. CONCLUSIONS :Established method is specific , precise and can be used for content determination of 4 components in Huanshao capsules.
期刊: 2020年第31卷第20期
作者: 周祥德,黄小兰,阳文武,黎海灵,周浓,邹隆琼,王立
英文作者: ZHOU Xiangde,HUANG Xiaolan,YANG Wenwu,LI Hailing,ZHOU Nong,ZOU Longqiong,WANG Li
关键字: 还少胶囊;高效液相色谱法;莫诺苷;马钱苷;松果菊苷;毛蕊花糖苷;含量测定
KEYWORDS: Huanshao capsules ;HPLC;Morroniside;Loganin;Echinacoside;Acteoside;Content determination
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