响应曲面法联合遗传算法优化天然低共熔溶剂提取肉苁蓉中苯乙醇苷类成分的工艺 点击下载
论文标题: 响应曲面法联合遗传算法优化天然低共熔溶剂提取肉苁蓉中苯乙醇苷类成分的工艺
英文标题:
中文摘要: 目的 优化天然低共熔溶剂(NADESs)提取肉苁蓉中苯乙醇苷类成分的工艺,为肉苁蓉的开发和利用提供参考。方法首先,以松果菊苷、毛蕊花糖苷和异毛蕊花糖苷的总提取效率为指标,筛选NADESs的类型;然后,在单因素实验基础上,通过响应曲面法优选最佳NADESs组成成分的摩尔比、NADESs含水量和液料比,并通过遗传算法对结果进行优化。以维生素C(VC)为阳性对照,从提取效率、体外抗氧化活性2个方面比较NADESs和传统溶剂(50%甲醇)的提取效果。结果最佳提取溶剂为由1,4-丁二醇和丙二酸组成的NADES-11。最优提取工艺为:NADES-11中1,4-丁二醇和丙二酸的摩尔比1∶2.5、NADES-11含水量18%、液料比30mL/g、提取时间30min、提取温度30℃。NADES-11的提取效率显著高于50%甲醇(P<0.05);NADES-11提取物对1,1-二苯基-2-三硝基苯肼自由基和羟基自由基的半数清除浓度(261.17、744.34µg/mL)均低于50%甲醇提取物(420.97、1175.12µg/mL),其抗坏血酸当量(17.19、360.80mgVC/g)高于50%甲醇提取物(10.67、228.54mgVC/g)。结论本研究优化的NADESs提取肉苁蓉中苯乙醇苷类成分的工艺环保、稳定、可行。
英文摘要: OBJECTIVE To optimize the extraction technology fo r phenylethanol glycosides from Cistanche deserticola by natural deep eutectic solvents (NADESs),and to provide reference for the development and utilization of C. deserticola . METHODS The optimal NADESs was selected using total extraction efficiency of echinacoside ,acteoside and isoacteoside as indexes. Based on single factor test ,response surface methodology was used to select the optimal NADESs molar ratio ,the optimal NADESs water content ,the optimal liquid-solid ratio ;and the results were optimized by genetic algorithm . Using vitamin C (VC) as positive control ,the extraction effects of NADESs and traditional solvent (50% methanol)were compared in respects of extraction efficiency and antioxidant activities. RESULTS The optimal extraction solution was NADES- 11 composed of 1, 4-butanediol and malonic acid. The optimal extraction technology was as follows as the molar ratio of 1,4-butanediol-malonic acid was 1 ∶ 2.5,water content of NADES- 11 was 18%,liquid-solid ratio was 30 mL/g,extraction time was 30 min and extraction temperature was 30 ℃. The extraction efficiency of NADES- 11 was significantly higher than that of 50% methanol(P<0.05). IC 50 values of NADES- 11 extract(261.17 and 744.34 µg/mL)to 1,1-diphenyl-2-trinitrophenylhydrazine radical and hydroxyl radical were all lower than those of 50% methanol extract (420.97 and 1 175.12 μg/mL). Ascorbic acid equivalent antioxidant capacity of Δ 基金项目 内蒙古自治区科技创新引导项目(No.00120209);内 NADES-11 extract(17.19 and 360.80 mg VC/g )was higher 蒙古自治区自然科学基金资助项目 (No.2021MS08011);内蒙古自治 than that of 50% methanol extract (10.67 and 228.54 mg 区医疗卫生科技计划项目(No.202201367);包头医学院“花蕾计划”项 VC/g). CONCLUSIONS The optimized extraction process of 目(No.HL2021046) phenylethanol glycosides from C. deserticola using NADESs is *第一作者 硕士研究生。研究方向:中蒙药药效成分。E-mail: environmental,stable and feasible. dongjiani369@126.com
期刊: 2022年第33卷第13期
作者: 董佳妮,赵龙山,薄彧坤,杨丹,杨雪苗,谭谊萌,安明,邬国栋
英文作者: DONG Jiani,ZHAO Longshan ,BO Yukun ,YANG Dan,YANG Xuemiao ,TAN Yimeng ,AN Ming,WU Guodong
关键字: 天然低共熔溶剂;肉苁蓉;苯乙醇苷类成分;响应曲面法;遗传算法;抗氧化活性;提取效率
KEYWORDS: natural deep eutectic solvents ; Cistanche
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