目的：建立测定大蒜中甾体皂苷含量的方法。方法：以对硝基苯甲酰氯为衍生化剂对大蒜中甾体皂苷进行柱前衍生化，

采用高效液相色谱法测定其含量。色谱柱为Shimadzu VP-ODS，流动相为乙腈-水（80 ∶20，V/V），流速为1.0 ml/min，检测波长为

254 nm，柱温为25 ℃，进样量为20 μl。结果：菝葜皂苷检测质量浓度线性范围为0～1.25 mg/ml（r＝0.999 0）；精密度、稳定性、重

复性试验的RSD＜2％；加样回收率为93.1％～96.8％（RSD＝1.56％，n＝6）。结论：该方法操作简便、稳定、重复性好，可用于大蒜

中甾体皂苷含量的测定。

OBJECTIVE：To establish a method for content determination of steroidal saponin in Allium sativum. METHODS：

Pre-column derivatization of steroidal saponin was performed by using the derivatization agent of nitro-benzoic acid-chlorine. And

HPLC was conducted to determine the content of steroidal saponin. The column was Shimadzu VP-ODS with mobile phase of acetonitrile

- water mixed solution（80 ∶20，V/V）at a flow rate of 1.0 ml/min，the detection wavelength was 254 nm，the column temperature

was 25 ℃，and the injection volume of 20 μl. RESULTS：The linear rang of sarsasapogenin was 0-1.25 mg/ml（r＝0.999 0）；

RSDs of precision，stability and reproducibility tests were lower than 2％；recovery was 93.1％-96.8％（RSD＝1.56％，n＝6）. CONCLUSIONS：

The method is simple，stable with good separation，and can be use for the content determination of steroidal saponin

in A. sativum.