HPLC法测定藏药榜嘎中大麦芽碱的含量 点击下载
论文标题: HPLC法测定藏药榜嘎中大麦芽碱的含量
英文标题:
中文摘要:

目的:建立测定藏药榜嘎中大麦芽碱含量的方法。方法:采用高效液相色谱法。色谱柱为Gemini-NX C18,流动相为乙

腈-0.25 mol/L 乙酸铵溶液(氨水调pH至9.5)(梯度洗脱),流速为1 ml/min,检测波长为230 nm,柱温为30 ℃,进样量为20 μl。结

果:大麦芽碱检测质量浓度线性范围为3.276~819 μg/ml(r=0.999 5);精密度、稳定性、重复性试验的RSD<2%;加样回收率为

96.21%~104.04%(RSD=1.23%,n=9)。结论:该方法操作简便、稳定、重复性好,可用于藏药榜嘎中大麦芽碱含量的测定。

英文摘要:

OBJECTIVE:To establish a method for the content determination of hordenine in Zang medicine Herba Aconiti.

METHODS:HPLC was performed on the column of Gemini-NX C18 with mobile phase of acetonitrile-0.25 mol/l Ammonium acetate

solution(ammonia water to adjust the pH to 9.5,gradient elution,at flow rate of 1 ml/min,the detection wavelength was 230

nm,the column temperature was 30 ℃,and the injection volume was 20 μl.RESULTS:The linear range of hordenine was 3.276-819

μg/ml(r=0.999 5);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 96.21%-104.04%

(RSD=1.23%,n=9). CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the content determination

of hordenine in Zang medicine Herba Aconiti.

期刊: 2016年第27卷第3期
作者: 卿大双,王欣,覃瑶,姚林才,王思平,杨勇,罗维早
英文作者: QING Dashuang,WANG Xin,QIN Yao,YAO Lincai,WANG Siping,YANG Yong,LUO Weizao
关键字: 榜嘎;高效液相色谱法;大麦芽碱;含量测定
KEYWORDS: Herba Aconiti;HPLC;Heteratisine;Content determination
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