HPLC法测定藤梨根中表儿茶素的含量 点击下载
论文标题: HPLC法测定藤梨根中表儿茶素的含量
英文标题:
中文摘要:

目的:建立测定藤梨根中表儿茶素含量的方法。方法:采用高效液相色谱法。色谱柱为Kromasil C18,流动相为乙

腈-0.2%乙酸溶液(15 ∶85,V/V),流速为1.0 ml/min,检测波长为210 nm,柱温为25 ℃,进样量为10 μl。结果:表儿茶素的检测质量

浓度线性范围为10.47~167.52 μg/ml(r=0.999 9);精密度、稳定性、重复性试验的RSD<2%;加样回收率为98.07%~101.71%,

RSD=1.39%(n=6)。结论:该方法操作简便、结果准确,适用于藤梨根中表儿茶素的含量测定。

英文摘要:

OBJECTIVE:To establish a method for the content determination of L-epicatechin in Actinidiae arguta. METHODS:

HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.2% Acetic acid solution(15 ∶85,V/V)at a

flow rate of 1.0 ml/min,detection wavelength was 210 nm,column temperature was 25 ℃,and the volume injection was 10 μl.

RESULTS:The linear range of L-epicatechin was 10.47-167.52 μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility

tests were lower than 2%;average recovery was 98.07%-101.71%(RSD=1.39%,n=6). CONCLUSIONS:The method is simple,

accurate and reliable,and suitable for the content determination of L-epicatechin in A. arguta.

期刊: 2016年第27卷第3期
作者: 赫军,马秉智,梁莹莹,王晓雪,刘晓
英文作者: HE Jun,MA Bingzhi,LIANG Yingying,WANG Xiaoxue,LIU Xiao
关键字: 高效液相色谱法;藤梨根;表儿茶素;含量测定
KEYWORDS: HPLC;Actinidia arguta;L-epicatechin;Content determination
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