目的：建立同时测定维舒膏中醋酸曲安奈德和维A酸含量的方法。方法：采用高效液相色谱法。色谱柱为Diamonsil

C18，流动相为甲醇-0.1％磷酸溶液（87 ∶13，V/V），检测波长为254 nm（0～5 min）和350 nm（5～20 min），流速为1.0 ml/min，柱温为

25 ℃，进样量为10 μl。结果：醋酸曲安奈德和维A酸的检测质量浓度线性范围分别为0.5～5、0.496～4.96 μg/ml（r＝0.999 9、0.999 8）；

精密度、稳定性、重复性试验的RSD＜1％；加样回收率分别为98.37％～100.03％、98.21％～100.38％，RSD分别为0.58％、0.78％

（n＝6）。结论：该方法简便、准确、快捷，适用于维舒膏中醋酸曲安奈德和维A酸的含量测定。

OBJECTIVE：To establish a method for the contents determination of triamcinolone acetonide acetate and tretinoin

in Dimensional ointment. METHODS：HPLC was performed on the column of Diamonsil C18 with mobile phase of methanol-0.1％

Phosphoric acid solution（87 ∶13，V/V），detection wavelength was 254 nm（0-5 min）and 350 nm（5-20 min），at a flow rate of 1.0

ml/min，column temperature was 25 ℃，and volume injection was 10 μl. RESULTS：The linear range was 0.5-5 μg/ml for triamcinolone

acetonide acetate（r＝0.999 9）and 4.96-49.6 μg/ml for tretinoin（r＝0.999 8）；RSDs of precision，stability and reproducibility

tests were lower than 1％；recoveries were 98.37％-100.03％（RSD＝0.58％，n＝6）and 98.21％-100.38％（RSD＝0.78％，n＝6）. CONCLUSIONS：

The method is simple，accurate and fast，and suitable for the contents determination of triamcinolone acetonide acetate

and tretinoin in Dimensional ointment.