HPLC法同时测定安脑丸中栀子苷、盐酸小檗碱和黄芩苷的含量 点击下载
论文标题: HPLC法同时测定安脑丸中栀子苷、盐酸小檗碱和黄芩苷的含量
英文标题:
中文摘要:

目的:建立同时测定安脑丸中栀子苷、盐酸小檗碱和黄芩苷含量的方法。方法:采用高效液相色谱法。色谱柱为Inertsil

ODS-3 C18,流动相为乙腈-0.2%磷酸溶液(梯度洗脱),流速为1.0 ml/min,柱温为30 ℃,检测波长为230 nm,进样量为10 μl。结果:栀子

苷、盐酸小檗碱和黄芩苷的检测进样量线性范围分别为0. 041 0~0.820 0、0. 037 6~0.752 0、0. 095 2~1.904 0 μg(r=0.999 9);加样回

收率分别为97.31%~100.41%、98.32%~101.76%、99.16%~101.30%,RSD分别为1.10%、1.20%、0.76%(n=6)。结论:该方法

简便、准确,可用于安脑丸的质量控制。

英文摘要:

OBJECTIVE:To establish a method for simultaneous determination of geniposide,berberine hydrochloride and baicalin

in Annao pill. METHODS:HPLC was performed on the column of Inertsil ODS-3 C18 with mobile phase of acetonitrile -

0.2% phosphoric acid solution(gradient elution)at a flow rate of 1.0 ml/min,the column temperature was 30 ℃,the detection

wavelength was 230 nm,and the injection volume was 10 μl. RESULTS:The linear range was 0. 041 0-0.820 0 μg for geniposide

(r=0.999 9),0.037 6-0.752 0 μg for berberine hydrochloride(r=0.999 9)and 0.095 2-1.904 0 μg for baicalin(r=0.999 9),respectively;

recoveries were 97.31% -100.41%(RSD=1.10% ,n=6),98.32% -101.76%(RSD=1.20% ,n=6) and 99.16% -101.30%

(RSD=0.76%,n=6),respectively. CONCLUSIONS:The method is simple and accurate,and can be used for the quality control

of Annao pill.

期刊: 2016年第27卷第3期
作者: 孙艳涛
英文作者: SUN Yantao
关键字: 安脑丸;栀子苷;盐酸小檗碱;黄芩苷;高效液相色谱法
KEYWORDS: Annao pill;Geniposide;Berberine hydrochloride;Baicalin;HPLC
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