HPLC法测定复方依沙吖啶软膏中乳酸依沙吖啶的含量 点击下载
论文标题: HPLC法测定复方依沙吖啶软膏中乳酸依沙吖啶的含量
英文标题:
中文摘要: 目的:建立测定复方依沙吖啶软膏中乳酸依沙吖啶含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent ZORBAX SB-C18,流动相为含0.1%辛烷磺酸钠溶液的磷酸盐缓冲液-乙腈(70 ∶ 30,V/V),流速为1.0 ml/min,检测波长为270 nm,柱温为30 ℃,进样量为10 μl。结果:乳酸依沙吖啶检测质量浓度线性范围为10.002~50.010 μg/ml(r=0.999 9);精密度、稳定性、重复性试验的RSD<1%;回收率为98.96%~100.36%,RSD=0.49%(n=9)。结论:该方法简便、灵敏、重复性好,可用于复方依沙吖啶软膏中乳酸依沙吖啶的含量测定。
英文摘要: OBJETCTIVE: To establish a method for the content determination of ethacridine lactate in Compound ethacridine ointment. METHODS: HPLC was performed on the column of Agilent ZORBAX SB-C18 with mobile phase of 0.1% Octanesulfonic acid sodium solution-acetonitrile (70 ∶ 30,V/V) at a flow rate of 1.0 ml/min, the detection wavelength was 270 nm, the column temperature was 30 ℃, and the injection volume was 10 μl. RESULTS: The linear range of ethacridine lactate was 10.002-50.010 μg/ml(r=0.999 9); RSDs of precision, stability and reproducibility tests were less than 1%; recovery was 98.96%-100.36% (RSD=0.49%,n=9). CONCLUSIONS: The method is simple, accurate and reproducible, and can be used for content determination of ethacridine lactate in Compound ethacridine ointment.
期刊: 2016年第27卷第15期
作者: 刘俊丽,田佳懿,段松冷,曾蔚欣,金锐,孙路路
英文作者: LIU Junli,TIAN Jiayi,DUAN Songleng,ZENG Weixin,JIN Rui,SUN Lulu
关键字: 复方依沙吖啶软膏;乳酸依沙吖啶;含量测定;高效液相色谱法
KEYWORDS: Compound ethacridine ointment; Ethacridine lactate; Content determination; HPLC
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