HPLC法测定八珍丸(浓缩丸)中芍药苷的含量 点击下载
论文标题: HPLC法测定八珍丸(浓缩丸)中芍药苷的含量
英文标题:
中文摘要: 目的:建立测定八珍丸(浓缩丸)中芍药苷含量的方法。方法: 采用高效液相色谱法。色谱柱为Wonda Sil C18,流动相为乙腈-1%磷酸(15 ∶ 85,V/V),流速为1.0 ml/min,检测波长为230 nm,柱温为30 ℃。结果:芍药苷检测进样量线性范围为0.219~1.32 μg(r=0.999 9);精密度、稳定性、重复性试验的RSD<1%;加样回收率为95.82%~101.82%(RSD=2.13%,n=6)。结论 :该方法操作简便、稳定、重复性好,可用于八珍丸(浓缩丸)中芍药苷含量的测定。
英文摘要: OBJECTIVE: To establish a method for the content determination of paeoniflorin in Bazhen pill (concentrated pill). METHODS: HPLC was performed on the column of wondasil C18 with mobile phase of acetonitrile -1% phosphoric acid (15 ∶ 85, V/V) at a flow rate of 1.0 ml/min, the detection wavelength was 230 nm, and column temperature was 30 ℃. RESULTS: The linear range of paeoniflorin was 0219-1.32 μg(r=0.999 9); RSDs of precision, stability and reproducibility tests were lower than 1%; recovery was 95.82%-101.82% (RSD=2.13%,n=6). CONCLUSIONS: The method is simple, stable and reproducible, and can be used for the content determination of Bazhen pill (concentrated pill).
期刊: 2016年第27卷第15期
作者: 黄卫娟,何秀云,刘杰,郑武娟,陈丽英
英文作者: HUANG Weijuan,HE Xiuyun,LIU Jie,ZHENG Wujuan,CHEN Liying
关键字: 八珍丸(浓缩丸);芍药苷;高效液相色谱法
KEYWORDS: Bazhen pill (concentrated pill); Paeoniflorin; HPLC
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