RP-HPLC法同时测定消炎利胆片中咖啡酸、异夏佛塔苷、夏佛塔苷的含量 点击下载
论文标题: RP-HPLC法同时测定消炎利胆片中咖啡酸、异夏佛塔苷、夏佛塔苷的含量
英文标题:
中文摘要: 目的:建立同时测定消炎利胆片中咖啡酸、异夏佛塔苷、夏佛塔苷含量的方法。方法:采用反相高效液相色谱法。色谱柱为Phenomenex C18,流动相为甲醇-0.1%磷酸水溶液(梯度洗脱),流速为0.8 ml/min,检测波长为334 nm,柱温为25 ℃,进样量为10 μl。结果:咖啡酸、异夏佛塔苷、夏佛塔苷的检测进样量线性范围分别为0.10~2.81、0.20~5.63、0.10~2.63 μg(r=0.999 9、0.999 9、0.999 9);精密度、稳定性、重复性试验的RSD<3%;加样回收率分别为96.3%~100.4%(RSD=1.59%,n=9)、97.3%~101.2%(RSD=1.28%,n=9)、96.6%~100.6%(RSD=1.39%,n=9)。结论:该方法简单灵敏、准确可靠、重复性好,可用于同时测定消炎利胆片中咖啡酸、异夏佛塔苷、夏佛塔苷的含量。
英文摘要: OBJECTIVE: To establish a method for the simultaneous determination of caffeic acid, isoschaftoside and schaftoside in Xiaoyan lidan tablet. METHODS: RP-HPLC was performed on the column of Phenomenex C18 with mobile phase of methanol - 0.1% phsphate acid (gradient elution) at flow rate of 0.8 ml/min, the detection wavelength was 334 nm, column temperature was 25 ℃, and the injection volume was 10 μl. RESULTS: The linear range was 0.10-2.81 μg for caffeic acid(r=0.999 9), 0.20-5.63 μg for isoschaftoside (r=0.999 9) and 0.10-2.63 μg for schaftoside(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 3%; recoveries were 96.3%-100.4%(RSD=1.59%,n=9), 97.3%-101.2% (RSD=1.28%,n=9) and 96.6%-100.6%(RSD=1.39%,n=9), respectively. CONCLUSIONS: The method is sensitive, accurate, reliable and reproducible, and can be used for the simultaneous determination of caffeic acid, isoschaftoside and schaftoside in Xiaoyan lidan tablet the quality control of Xiaoyan lidan tablet.
期刊: 2016年第27卷第18期
作者: 张小梅
英文作者: ZHANG Xiaomei
关键字: 反相高效液相色谱法;消炎利胆片;咖啡酸、异夏佛塔苷;夏佛塔苷;含量测定
KEYWORDS: RP-HPLC; Xiaoyan lidan tablet; Caffeic acid; Isoschaftoside; Schaftoside; Content determination
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