UPLC法同时测定胃苏颗粒中4种成分的含量 点击下载
论文标题: UPLC法同时测定胃苏颗粒中4种成分的含量
英文标题:
中文摘要: 目的:建立同时测定胃苏颗粒中芸香柚皮苷、柚皮苷、橙皮苷和新橙皮苷含量的方法。方法:采用超高效液相色谱法。色谱柱为ACQUITY UPLC BEH C18,流动相为乙腈-0.2%磷酸(梯度洗脱),流速为0.40 ml/min,检测波长为284 nm,柱温为30 ℃,进样量为1 μl。结果:芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷的检测质量浓度线性范围分别为9.38~93.75 μg/ml(r=0.999 7)、32.25~322.50 μg/ml(r=0.999 7)、11.25~112.50 μg/ml(r=0.999 9)、11.88~118.75 μg/ml(r=0.999 8);定量限分别为20、18、18、18 ng,检测限分别为6、5、5、5 ng;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为96.24%~103.12%(RSD=2.45%,n=6)、98.43%~102.10%(RSD=1.42%,n=6)、96.10%~101.41%(RSD=2.07%,n=6)、95.57%~99.06%(RSD=1.44%,n=6)。结论:该方法快速、高效,适用于同时测定胃苏颗粒中芸香柚皮苷、柚皮苷、橙皮苷和新橙皮苷的含量。
英文摘要: OBJECTIVE: To establish a method for the simultaneous determination of narirutin, naringin, hesperiden and neohesperiden in Weisu granule. METHODS: UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of acetonitrile-0.2% Phosphoric acid aqueous(gradient elution) at a flow rate of 0.40 ml/min, the detection wavelength was 284 nm, the column temperature was 30 ℃, and the injection volume was 1 μl. RESULTS: The linear range was 9.38-93.75 μg/ml for narirutin(r=0.999 7), 32.25-322.50 μg/ml for naringin(r=0.999 7), 11.25-112.50 μg/ml for hesperiden(r=0.999 9) and 11.88-118.75 μg/ml for neohesperidin(r=0.999 8);limits of quantitation were 20 ng, 18 ng,18 ng and 18 ng,the limits of detection were 6 ng,5 ng ,5 ng and 5 ng, respectively; RSDs of precision, stability and reproducibility tests were lower than 2.0%; recoveries were 96.24%-103.12% (RSD=2.45%,n=6), 98.43%-102.10% (RSD=1.42%,n=6), 96.10%-101.41% (RSD=2.07%,n=6) and 95.57%-99.06% (RSD=1.44%,n=6), respectively. CONCLUSIONS: The method is rapid and efficient, and suitable for the simultaneous determination of narirutin, naringin, hesperiden and neohesperiden in Weisu granule.
期刊: 2016年第27卷第24期
作者: 郭殷锐,张广唱,王剑
英文作者: GUO Yinrui,ZHANG Guangchang,WANG Jian
关键字: 超高效液相色谱法;胃苏颗粒;芸香柚皮苷;橙皮苷;新橙皮苷;柚皮苷;含量测定
KEYWORDS: UPLC; Weisu granule; Narirutin; Hesperiden; Neohesperidin; Naringin; Content determination
总下载数: 81次
本日下载数: 2次
本月下载数: 81次
文件大小: 619.60Kb

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!