HPLC-ELSD法测定琥乙红霉素片中琥乙红霉素的含量 点击下载
论文标题: HPLC-ELSD法测定琥乙红霉素片中琥乙红霉素的含量
英文标题:
中文摘要: 目的:建立高效液相色谱-蒸发光散射检测器法测定琥乙红霉素片中琥乙红霉素含量的方法。方法:色谱柱为Dikma-C18,流动相为乙腈-0.05%二乙胺(70 ∶ 30,V/V),流速为1.0 ml/min,柱温为30 ℃,进样量为10 μl。结果:琥乙红霉素检测进样量线性范围为1.216~7.296 μg(r=0.999 2);定量限为0.304 μg,检测限为0.076 μg;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率为97.59%~104.19%(RSD=1.71%,n=6)。结论 :该方法操作简便、结果准确、灵敏度高、重复性好,可用于测定琥乙红霉素片中琥乙红霉素的含量。
英文摘要: OBJECTIVE: To establish a method for the content determination of erythromycin ethylsuccinate in Erythromycin ethylsuccinate tablet. METHODS:The column of Dikma-C18 with mobile phase of acetonitrile-0.05% diethylamine(70 ∶ 30,V/V) at a flow rate of 1.0 ml/min, column temperature was 30 ℃, and the sample size was 10 μl. RESULTS:The linear range of erythromycin ethylsuccinate was 1.216-7.296 μg (r=0.999 2); limit of quantitation was 0.304 μg, the limits of detection was 0.076 μg, respectively;the RSDs of precision, stability and reproducibility tests were no more than 2.0%, the average recoveries was 97.59%-104.19%(RSD=1.71%,n=6). CONCLUSIONS: The method is simple and accurate with high sensitivity and reproducibility, and can be used for the content determination of Erythromycin ethylsuccinate tablet.
期刊: 2016年第27卷第33期
作者: 李志得,张彩云,汪霞
英文作者: LI Zhide,ZHANG Caiyun,WANG Xia
关键字: 琥乙红霉素片;高效液相色谱法;琥乙红霉素;含量测定
KEYWORDS: Erythromycin ethylsuccinate tablet; HPLC; Erythromylin ethyisuccinate; Content determination
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