桑叶药材中总黄酮含量的测定 点击下载
论文标题: | 桑叶药材中总黄酮含量的测定 |
英文标题: | |
中文摘要: | 目的:建立测定桑叶中总黄酮含量的方法。方法:采用紫外可见分光光度法。以芦丁为对照品,采用亚硝酸钠-硝酸铝-氢氧化钠显色法,在510 nm处测定桑叶中总黄酮的含量。结果:芦丁检测质量浓度线性范围为0.031 2~0.156 mg/ml(r=0.999 9);精密度、稳定性、重复性试验的RSD<2%;加样回收率为95.7%~101.0%(RSD=2.1%,n=6)。结论:该方法操作简便、稳定、重复性好,可用于桑叶中总黄酮含量的测定。 |
英文摘要: | OBJECTIVE:To establish a method for the content determination of total flavonoids in Morus alba. METHODS: UV-visible spectrophometry was performed with Al(NO2)3-NaNO2-NaOH color-test at the wavelength of 510 nm with the reference of rutin. RESULTS: The linear range of rutin was 0.031 2-0.156 mg/ml (r=0.999 9); RSDs of precision, stability and reproducibility tests were lower than 2%; recovery was 95.7%-101.0% (RSD=2.1%,n=6). CONCLUSIONS: The method is simple, stable and reproducible, and can be used for the content determination of total flavonoids in M. alba. |
期刊: | 2016年第27卷第6期 |
作者: | 吕丹,王海波,邸学,张振秋 |
英文作者: | LYU Dan,WANG Haibo,DI Xue,ZHANG Zhenqiu |
关键字: | 桑叶;总黄酮;紫外可见分光光度法 |
KEYWORDS: | Morus alba; Total flavonoids; UV-visible spectrophometry |
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