紫肉甘薯药材的质量标准研究 点击下载
论文标题: | 紫肉甘薯药材的质量标准研究 |
英文标题: | |
中文摘要: | 目的:建立紫肉甘薯的质量标准。方法:检测药材的显微特征;采用薄层色谱法(TLC)对药材进行定性鉴别;测定药材水分、灰分、重金属和浸出物量;采用高效液相色谱法测定药材中氯化矢车菊素的含量:色谱柱为Alltima C18,流动相为乙腈-0.1%三氟乙酸溶液(80 ∶ 20,V/V) ,流速为1.0 ml/min,检测波长为538 nm,柱温为30 ℃。结果:药材粉末显微鉴别图谱清晰。紫肉甘薯的TLC图斑点清晰,分离良好。药材水分为8.5%~11.0%,总灰分为2.0%~2.9%,铅含量<5 ppm,浸出物为15.6%~22.3%。氯化矢车菊素检测进样量线性范围为0.043~0.215 μg(r=0.999 8);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率为95.50%~99.65%(RSD=1.35%,n=9)。结论:该研究所建标准可用于紫肉甘薯的质量控制。 |
英文摘要: | OBJECTIVE: To establish the quality standard for Solanum tuberdsm. METHODS: The microscopic characteristics were detected; TLC was used for qualitative identification; moisture, ash, heavy metals and the amount of extract were determined; HPLC was conducted for content determination of oxidized cyanidin: the column was Alltima C18 with mobile phase of acetonitrile-0.1% trifluoroacetic acid (80 ∶ 20,V/V) at a flow rate of 1.0 ml/min, detection wavelength was 538 nm, column temperature was 30℃. RESULTS: It showed clear microscopic identification map. S. tuberdsm TLC had clear spots and well separated. The water content was 8.5%-11.0%; total ash was 2.0%-2.9%; Pb was lower than 5 ppm and alcohol-soluble extracts was 15.6%-22.3%. The linear range of oxidized cyanidin was 0.043-0.215 μg (r=0.999 8); RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 95.50%-99.65%(RSD=1.35%,n=9). CONCLUSIONS: The established method can be used for the quality control of S. tuberdsm. |
期刊: | 2016年第27卷第30期 |
作者: | 王路平,王文成,田荣,姜启晓,王春波,隋忠国 |
英文作者: | WANG Luping,WANG Wencheng,TIAN Rong,JIANG Qixiao,WANG Chunbo,SUI Zhongguo |
关键字: | 紫肉甘薯;氯化矢车菊素;薄层色谱法;高效液相色谱法;质量标准 |
KEYWORDS: | Solanum tuberdsm; Oxidized cyaniding; TLC; HPLC; Quality standard |
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