基于低共熔溶剂的翻白草中黄酮类成分的提取工艺研究 点击下载
| 论文标题： | 基于低共熔溶剂的翻白草中黄酮类成分的提取工艺研究 |
| 英文标题： | |
| 中文摘要： | 目的 优化翻白草中黄酮类成分的提取工艺。方法以低共熔溶剂（DESs）为溶剂制备供试品溶液，采用超高效液相色谱测定芦丁、槲皮苷和山柰酚的含量；采用层次分析-客观权重赋权综合赋权法对上述3种黄酮类成分的含量进行综合赋权并计算综合评分，以综合评分为指标筛选最佳DESs；同时，以综合评分为响应值、液料比、超声时间、超声温度为指标，采用Box-Behnken响应面法优化翻白草中黄酮类成分的提取工艺并进行验证。结果芦丁、檞皮苷、山柰酚含量的综合权重系数分别为0.6467、0.2075、0.1458。最佳DESs为含水量40%的氯化胆碱-乳酸（摩尔比1∶4），最优提取工艺为液料比25∶1、超声时间40min、超声温度50℃。经验证，3批样品的平均综合评分为99.28分，RSD为0.54%（n＝3）。结论本研究基于DESs所得翻白草中黄酮类成分的最优提取工艺稳定、可行。 |
| 英文摘要： | OBJECTIVE To optimize the extraction process of flavonoids from Potentilla discolor .METHODS Deep eutectic solvents （DESs） were used as the extraction solvent to prepare test sample solutions. The contents of rutin， quercitrin， and kaempferol were determined by ultra-performance liquid chromatography. The analytic hierarchy process combined with the criteria importance through intercriteria correlation weighting method was employed to assign comprehensive weights to the contents of the three flavonoids， and calculate the comprehensive score. The optimal DESs were screened based on the comprehensive score.Mean while， using the comprehensive score as the response variable and the liquid-to-solid ratio， ultrasonic time， and urltrasonic temperature as the influencing factors， the extraction technology of flavonoids from P.discolor was optimized by Box-Behnken response surface methodology and subsequently validated.RESULTS The comprehensive weight coefficients of rutin， quercitrin， and kaempferol were 0.646 7， 0.207 5， and 0.145 8， respectively. The optimal DESs were choline chloride-lactic acid （molar ratio 1∶4） with a water content of 40%. The optimal extraction conditions were determined as follows： liquid-to-solid ratio of 25∶1， ultrasonic time of 40 min， and ultrasonic temperature of 50 ℃. The validation results showed that the average comprehensive score of three batches of samples was 99.28， with an RSD of 0.54% （ n ＝3）.CONCLUSIONS The optimal extraction technology of flavonoids from P. discolor based on DESs obtained in this study was stable and feasible. |
| 期刊： | 2026年第37卷第16期 |
| 作者： | 刘斌；杨臣逸；邵美玲；黄良永；高艳果；王雪芹；吕光辉；胡倩；叶方 |
| 英文作者： | LIU Bin，YANG Chenyi， SHAO Meiling，HUANG Liangyong，GAO Yanguo，WANG Xueqin，LYU Guanghui，HU Qian，YE Fang |
| 关键字： | 翻白草；黄酮类；低共熔溶剂；Box-Behnken响应面法；层次分析；客观权重赋权；综合赋权 |
| KEYWORDS： | Potentilla discolor;Flavonoids;deep eutectic solvents;Box-Behnken response surface methodology;AHP;CRITIC;combined weighting |
| 总下载数： | 81次 |
| 本日下载数： | 2次 |
| 本月下载数： | 81次 |
| 文件大小： | 619.60Kb |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！