论文标题: | 改进HPLC法同时测定注射用替卡西林钠克拉维酸钾中两种主成分的含量 |
英文标题: | |
中文摘要: | 目的:改进测定注射用替卡西林钠克拉维酸钾中两种主成分含量的方法。方法:采用高效液相色谱法。色谱柱为Waters XBridgeTM C18,流动相为0.01 mol/L磷酸氢二铵溶液(用磷酸调节pH至7.0)-甲醇(80 ∶ 20,V/V),流速为1.0 mL/min,检测波长为220 nm,柱温为30 ℃,进样量为 20 μL。结果:替卡西林和克拉维酸的检测质量浓度线性范围分别为1.95~195.22、0.12~12.18 μg/mL(r=0.999 9);精密度、稳定性(4 ℃下放置)、重复性试验的RSD<1.0%;替卡西林和克拉维酸的加样回收率分别为99.3%~100.5%、99.2%~101.0%,RSD分别为0.4%、0.7%(n=9)。结论:该方法简便快速、准确可靠,可用于注射用替卡西林钠克拉维酸钾中两种主成分含量的同时测定。 |
英文摘要: | OBJECTIVE: To improve the method for the determination of 2 main components in Ticarcillin disodium and potassium clavulanate for injection. METHODS: HPLC was performed on the column of Waters XBridgeTM C18 with mobile phase of 0.01 mol/L ammonium dibasic phosphate solution (pH 7.0)-menthol (80 ∶ 20,V/V) at a flow rate of 1.0 mL/min, the detection wavelength was 220 nm, column temperature was 30 ℃, and injection volume was 20 μL. RESULTS: The linear range was 1.95-195.22 μg/mL for ticarcillin (r=0.999 9) and 0.12-12.18 μg/mL for clavulanate(r=0.999 9); RSDs of precision, stability (under 4 ℃) and reproducibility tests were lower than 1.0%;recoveries were 99.3%-100.5%(RSD=0.4%,n=9) and 99.2%- 101.0% (RSD=0.7%,n=9). CONCLUSIONS: The method is rapid, accurate and reliable, and can be used for the determination of 2 main components in Ticarcillin disodium and potassium clavulanate for injection. |
期刊: | 2017年第28卷第6期 |
作者: | 王茉莉,张菁,张少萌,张兰桐 |
英文作者: | WANG Moli,ZHANG Jing,ZHANG Shaomeng,ZHANG Lantong |
关键字: | 高效液相色谱法;替卡西林;克拉维酸;注射用替卡西林钠克拉维酸钾;含量测定 |
KEYWORDS: | HPLC; Ticarcillin; Clavulanate; Ticarcillin disodium and potassium clavulanate for injection; Content determination |
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